Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

Jan W Buikema; Soah Lee; William R Goodyer; Renee G Maas; Orlando Chirikian; Guang Li; Yi Miao; Sharon L Paige; Daniel Lee; Haodi Wu; David T Paik; Siyeon Rhee; Lei Tian; Francisco X Galdos; Nazan Puluca; Benjamin Beyersdorf; James Hu; Aimee Beck; Sneha Venkamatran; Srilatha Swami; Paul Wijnker; Maike Schuldt; Larissa M Dorsch; Alain van Mil; Kristy Red-Horse; Joy Y Wu; Caroline Geisen; Michael Hesse; Vahid Serpooshan; Stefan Jovinge; Bernd K Fleischmann; Pieter A Doevendans; Jolanda van der Velden; K Christopher Garcia; Joseph C Wu; Joost P G Sluijter; Sean M Wu

doi:10.1016/j.stem.2020.06.001

Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

Jan W Buikema
, Soah Lee
, William R Goodyer
, Renee G Maas
, Orlando Chirikian
, Guang Li
, Yi Miao
, Sharon L Paige
, Daniel Lee
, Haodi Wu
, David T Paik
, Siyeon Rhee
, Lei Tian
, Francisco X Galdos
, Nazan Puluca
, Benjamin Beyersdorf
, James Hu
, Aimee Beck
, Sneha Venkamatran
, Srilatha Swami

Paul Wijnker, Maike Schuldt, Larissa M Dorsch, Alain van Mil, Kristy Red-Horse, Joy Y Wu, Caroline Geisen, Michael Hesse, Vahid Serpooshan, Stefan Jovinge, Bernd K Fleischmann, Pieter A Doevendans, Jolanda van der Velden, K Christopher Garcia, Joseph C Wu, Joost P G Sluijter, Sean M Wu

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.

Original language	English
Pages (from-to)	50-63.e5
Journal	Cell stem cell
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.stem.2020.06.001
Publication status	Published - 2 Jul 2020

Keywords

GSK3
Wnt signaling
cardiomyocytes
expansion
induced pluripotent stem cells
maturation
proliferation

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10.1016/j.stem.2020.06.001

1-s2.0-S193459092030268X-mainFinal published version, 6.75 MBLicence: Taverne

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Buikema, J. W., Lee, S., Goodyer, W. R., Maas, R. G., Chirikian, O., Li, G., Miao, Y., Paige, S. L., Lee, D., Wu, H., Paik, D. T., Rhee, S., Tian, L., Galdos, F. X., Puluca, N., Beyersdorf, B., Hu, J., Beck, A., Venkamatran, S., ... Wu, S. M. (2020). Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes. Cell stem cell, 27(1), 50-63.e5. https://doi.org/10.1016/j.stem.2020.06.001

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title = "Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes",

abstract = "Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.",

keywords = "GSK3, Wnt signaling, cardiomyocytes, expansion, induced pluripotent stem cells, maturation, proliferation",

author = "Buikema, \{Jan W\} and Soah Lee and Goodyer, \{William R\} and Maas, \{Renee G\} and Orlando Chirikian and Guang Li and Yi Miao and Paige, \{Sharon L\} and Daniel Lee and Haodi Wu and Paik, \{David T\} and Siyeon Rhee and Lei Tian and Galdos, \{Francisco X\} and Nazan Puluca and Benjamin Beyersdorf and James Hu and Aimee Beck and Sneha Venkamatran and Srilatha Swami and Paul Wijnker and Maike Schuldt and Dorsch, \{Larissa M\} and \{van Mil\}, Alain and Kristy Red-Horse and Wu, \{Joy Y\} and Caroline Geisen and Michael Hesse and Vahid Serpooshan and Stefan Jovinge and Fleischmann, \{Bernd K\} and Doevendans, \{Pieter A\} and \{van der Velden\}, Jolanda and Garcia, \{K Christopher\} and Wu, \{Joseph C\} and Sluijter, \{Joost P G\} and Wu, \{Sean M\}",

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year = "2020",

month = jul,

day = "2",

doi = "10.1016/j.stem.2020.06.001",

language = "English",

volume = "27",

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journal = "Cell stem cell",

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Buikema, JW, Lee, S, Goodyer, WR, Maas, RG, Chirikian, O, Li, G, Miao, Y, Paige, SL, Lee, D, Wu, H, Paik, DT, Rhee, S, Tian, L, Galdos, FX, Puluca, N, Beyersdorf, B, Hu, J, Beck, A, Venkamatran, S, Swami, S, Wijnker, P, Schuldt, M, Dorsch, LM, van Mil, A, Red-Horse, K, Wu, JY, Geisen, C, Hesse, M, Serpooshan, V, Jovinge, S, Fleischmann, BK, Doevendans, PA, van der Velden, J, Garcia, KC, Wu, JC, Sluijter, JPG & Wu, SM 2020, 'Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes', Cell stem cell, vol. 27, no. 1, pp. 50-63.e5. https://doi.org/10.1016/j.stem.2020.06.001

TY - JOUR

T1 - Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

AU - Buikema, Jan W

AU - Lee, Soah

AU - Goodyer, William R

AU - Maas, Renee G

AU - Chirikian, Orlando

AU - Li, Guang

AU - Miao, Yi

AU - Paige, Sharon L

AU - Lee, Daniel

AU - Wu, Haodi

AU - Paik, David T

AU - Rhee, Siyeon

AU - Tian, Lei

AU - Galdos, Francisco X

AU - Puluca, Nazan

AU - Beyersdorf, Benjamin

AU - Hu, James

AU - Beck, Aimee

AU - Venkamatran, Sneha

AU - Swami, Srilatha

AU - Wijnker, Paul

AU - Schuldt, Maike

AU - Dorsch, Larissa M

AU - van Mil, Alain

AU - Red-Horse, Kristy

AU - Wu, Joy Y

AU - Geisen, Caroline

AU - Hesse, Michael

AU - Serpooshan, Vahid

AU - Jovinge, Stefan

AU - Fleischmann, Bernd K

AU - Doevendans, Pieter A

AU - van der Velden, Jolanda

AU - Garcia, K Christopher

AU - Wu, Joseph C

AU - Sluijter, Joost P G

AU - Wu, Sean M

PY - 2020/7/2

Y1 - 2020/7/2

N2 - Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.

AB - Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.

KW - GSK3

KW - Wnt signaling

KW - cardiomyocytes

KW - expansion

KW - induced pluripotent stem cells

KW - maturation

KW - proliferation

UR - https://www.scopus.com/pages/publications/85087025365

U2 - 10.1016/j.stem.2020.06.001

DO - 10.1016/j.stem.2020.06.001

M3 - Article

C2 - 32619518

SN - 1934-5909

VL - 27

SP - 50-63.e5

JO - Cell stem cell

JF - Cell stem cell

IS - 1

ER -

Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

Abstract

Keywords

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