Within-patient comparison between [Ga-68]Ga-tilmanocept PET/CT lymphoscintigraphy and [Tc-99m]Tc-tilmanocept lymphoscintigraphy for sentinel lymph node detection in oral cancer: a pilot study: a pilot study

Purpose: To compare sentinel lymph node (SLN) identification using [⁶⁸Ga]Ga-tilmanocept PET/CT lymphoscintigraphy to [^99mTc]Tc-tilmanocept lymphoscintigraphy (including SPECT/CT) in early-stage oral cancer. Furthermore, to assess whether reliable intraoperative SLN localization can be performed with a conventional portable gamma-probe using [^99mTc]Tc-tilmanocept without the interference of [⁶⁸Ga]Ga-tilmanocept in these patients. Methods: This prospective within-patient comparison pilot study evaluated SLN identification by [⁶⁸Ga]Ga-tilmanocept PET/CT lymphoscintigraphy compared to conventional lymphoscintigraphy using [^99mTc]Tc-tilmanocept (~ 74 MBq) in 10 early-stage oral cancer patients scheduled for SLN biopsy. After conventional [^99mTc]Tc-tilmanocept lymphoscintigraphy, patients underwent peritumoral administration of [⁶⁸Ga]Ga-tilmanocept (~ 10 MBq) followed by PET/CT acquisition initiated 15 min after injection. Intraoperative SLN localization was performed under conventional portable gamma-probe guidance the next day; the location of harvested SLNs was correlated to both lymphoscintigraphic images in each patient. Results: A total of 24 SLNs were identified by [^99mTc]Tc-tilmanocept lymphoscintigraphy, all except one were also identified by [⁶⁸Ga]Ga-tilmanocept PET/CT lymphoscintigraphy. [⁶⁸Ga]Ga-tilmanocept PET/CT lymphoscintigraphy identified 4 additional SLNs near the injection site, of which two harbored metastases. Lymphatic vessels transporting [⁶⁸Ga]Ga-tilmanocept were identified by PET/CT lymphoscintigraphy in 80% of patients, while draining lymphatic vessels were visualized by [^99mTc]Tc-tilmanocept lymphoscintigraphy in 20% of patients. Of the 33 SLNs identified by [⁶⁸Ga]Ga-tilmanocept PET/CT lymphoscintigraphy, 30 (91%) were intraoperatively localized under conventional gamma-probe guidance. Conclusion: [⁶⁸Ga]Ga-tilmanocept PET/CT lymphoscintigraphy provided more accurate identification of SLNs and improved visualization of lymphatic vessels compared to [^99mTc]Tc-tilmanocept lymphoscintigraphy. When combined with peritumoral administration of [^99mTc]Tc-tilmanocept, SLNs detected by [⁶⁸Ga]Ga-tilmanocept PET/CT lymphoscintigraphy can be reliably localized during surgery under conventional gamma-probe guidance.