VSTM1-v2 does not drive human Th17 cell differentiation: A replication study

Helen J. von Richthofen; Florianne M.J. Hafkamp; Anouk van Haperen; Esther C. de Jong; Linde Meyaard

doi:10.1371/journal.pone.0284404

VSTM1-v2 does not drive human Th17 cell differentiation: A replication study

Helen J. von Richthofen, Florianne M.J. Hafkamp, Anouk van Haperen, Esther C. de Jong, Linde Meyaard^*

^*Corresponding author for this work

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Abstract

Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune inhibitory receptor expressed on human myeloid cells. We previously showed that dendritic cell (DC)-driven Th17 cell differentiation of human naive CD4+ T cells requires presence of neutrophils, which is inhibited by SIRL-1 ligation. VSTM1-v2 is a soluble isoform of SIRL-1, which was previously proposed to function as a Th17 polarizing cytokine. Here, we investigated the effect of VSTM1-v2 on DC-driven Th17 cell development. Neutrophils induced DC-driven Th17 cell differentiation, which was not enhanced by VSTM1-v2. Similarly, we found no effect of VSTM1-v2 on cytokine-driven Th17 cell development. Thus, our results do not support a role for VSTM1-v2 in Th17 cell differentiation.

Original language	English
Article number	e0284404
Journal	PLoS ONE
Volume	18
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0284404
Publication status	Published - Apr 2023

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title = "VSTM1-v2 does not drive human Th17 cell differentiation: A replication study",

abstract = "Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune inhibitory receptor expressed on human myeloid cells. We previously showed that dendritic cell (DC)-driven Th17 cell differentiation of human naive CD4+ T cells requires presence of neutrophils, which is inhibited by SIRL-1 ligation. VSTM1-v2 is a soluble isoform of SIRL-1, which was previously proposed to function as a Th17 polarizing cytokine. Here, we investigated the effect of VSTM1-v2 on DC-driven Th17 cell development. Neutrophils induced DC-driven Th17 cell differentiation, which was not enhanced by VSTM1-v2. Similarly, we found no effect of VSTM1-v2 on cytokine-driven Th17 cell development. Thus, our results do not support a role for VSTM1-v2 in Th17 cell differentiation.",

author = "{von Richthofen}, {Helen J.} and Hafkamp, {Florianne M.J.} and {van Haperen}, Anouk and {de Jong}, {Esther C.} and Linde Meyaard",

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AU - von Richthofen, Helen J.

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AU - van Haperen, Anouk

AU - de Jong, Esther C.

AU - Meyaard, Linde

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AB - Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune inhibitory receptor expressed on human myeloid cells. We previously showed that dendritic cell (DC)-driven Th17 cell differentiation of human naive CD4+ T cells requires presence of neutrophils, which is inhibited by SIRL-1 ligation. VSTM1-v2 is a soluble isoform of SIRL-1, which was previously proposed to function as a Th17 polarizing cytokine. Here, we investigated the effect of VSTM1-v2 on DC-driven Th17 cell development. Neutrophils induced DC-driven Th17 cell differentiation, which was not enhanced by VSTM1-v2. Similarly, we found no effect of VSTM1-v2 on cytokine-driven Th17 cell development. Thus, our results do not support a role for VSTM1-v2 in Th17 cell differentiation.

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VSTM1-v2 does not drive human Th17 cell differentiation: A replication study

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