TY - JOUR
T1 - Use of IP-10 detection in dried plasma spots for latent tuberculosis infection diagnosis in contacts via mail
AU - Villar-Hernández, R.
AU - Latorre, I.
AU - De Souza-Galvão, M. L.
AU - Jiménez, M. A.
AU - Ruiz-Manzano, J.
AU - Pilarte, J.
AU - García-García, E.
AU - Muriel-Moreno, B.
AU - Cantos, A.
AU - Altet, N.
AU - Millet, J. P.
AU - González-Díaz, Y.
AU - Molina-Pinargote, I.
AU - Prat, C.
AU - Ruhwald, M.
AU - Domínguez, J.
N1 - Funding Information:
This study was supported by the grants from: (i) the Fundació Catalana de Pneumologia (FUCAP), (ii) the Instituto de Salud Carlos III (PI 13/01546, PI/16/01912), integrated in the Plan Nacional de I + D + I and cofunded by the ISCIII-Subdirección General de Evaluación and the Fondo Europeo de Desarrollo Regional (FEDER), (iii) and CERCA Programme/Generalitat de Catalunya. José Domínguez is a researcher funded by the Miguel Servet programme of the Instituto de Salud Carlos III.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-γ produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management.
AB - The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-γ produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management.
UR - https://www.scopus.com/pages/publications/85062588812
U2 - 10.1038/s41598-019-40778-1
DO - 10.1038/s41598-019-40778-1
M3 - Article
C2 - 30850687
AN - SCOPUS:85062588812
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 3943
ER -