TY - JOUR
T1 - Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
AU - Boero, Elena
AU - Brinkman, Iris
AU - Juliet, Thessely
AU - van Yperen, Eline
AU - van Strijp, Jos A.G.
AU - Rooijakkers, Suzan H.M.
AU - van Kessel, Kok P.M.
N1 - Funding Information:
We thank Rita Cruz for the critical reading of the manuscript.
Publisher Copyright:
© Copyright © 2021 Boero, Brinkman, Juliet, van Yperen, van Strijp, Rooijakkers and van Kessel.
Copyright © 2021 Boero, Brinkman, Juliet, van Yperen, van Strijp, Rooijakkers and van Kessel.
PY - 2021/2/19
Y1 - 2021/2/19
N2 - Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-throughput flow cytometry assay to quantify phagocytosis of S. aureus by human neutrophils. We show that effective phagocytic uptake of S. aureus is greatly enhanced by opsonization, i.e. the tagging of microbial surfaces with plasma-derived host proteins like antibodies and complement. Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies.
AB - Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-throughput flow cytometry assay to quantify phagocytosis of S. aureus by human neutrophils. We show that effective phagocytic uptake of S. aureus is greatly enhanced by opsonization, i.e. the tagging of microbial surfaces with plasma-derived host proteins like antibodies and complement. Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies.
KW - flow cytometry
KW - human
KW - neutrophils
KW - phagocytosis
KW - Staphylococcus aureus
KW - Complement Activation
KW - Humans
KW - Cells, Cultured
KW - Staphylococcus aureus/immunology
KW - Complement System Proteins/immunology
KW - Antibodies, Bacterial/immunology
KW - Neutrophils/immunology
KW - Flow Cytometry
KW - High-Throughput Screening Assays
KW - Immune Evasion
KW - Time Factors
KW - Bacteriological Techniques
KW - Opsonin Proteins/immunology
KW - Staphylococcal Infections/immunology
KW - Phagocytosis
UR - http://www.scopus.com/inward/record.url?scp=85102261821&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2021.635825
DO - 10.3389/fimmu.2021.635825
M3 - Article
C2 - 33679791
AN - SCOPUS:85102261821
SN - 1664-3224
VL - 12
SP - 1
EP - 15
JO - Frontiers in Immunology
JF - Frontiers in Immunology
M1 - 635825
ER -