Upstream AUG codons in the simian immunodeficiency virus SIVmac239 genome regulate Rev and Env protein translation

Gisela J van der Velden; Bep Klaver; Atze T Das; Ben Berkhout

doi:10.1128/JVI.01532-12

Upstream AUG codons in the simian immunodeficiency virus SIVmac239 genome regulate Rev and Env protein translation

Gisela J van der Velden, Bep Klaver, Atze T Das, Ben Berkhout

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The mRNAs encoding the Rev and Env proteins of simian immunodeficiency virus (SIV) are unique because upstream translation start codons are present that may modulate the expression of these viral proteins. This is true for the regular mRNAs, but we also report novel mRNA splicing variants that encode up to five upstream AUG (uAUG) codons. Their influence on Rev and Env translation was measured by mutational inactivation in reporter constructs and in the SIVmac239 strain. An intricate regulatory mechanism was disclosed that allows the virus to express a balanced amount of these two proteins. This insight also allows the design of vector constructs that efficiently express these proteins.

Original language	English
Pages (from-to)	12362-71
Number of pages	10
Journal	Journal of Virology
Volume	86
Issue number	22
DOIs	https://doi.org/10.1128/JVI.01532-12
Publication status	Published - Nov 2012
Externally published	Yes

Keywords

Alternative Splicing
Codon
Codon, Initiator
Gene Products, env/genetics
Gene Products, rev/genetics
Genes, Reporter
Genome, Viral
HEK293 Cells
Humans
Leukocytes, Mononuclear/virology
Models, Genetic
Mutation
Open Reading Frames
RNA Splicing
RNA, Messenger/metabolism
Sequence Analysis, DNA
Simian Immunodeficiency Virus/genetics

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10.1128/JVI.01532-12

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title = "Upstream AUG codons in the simian immunodeficiency virus SIVmac239 genome regulate Rev and Env protein translation",

abstract = "The mRNAs encoding the Rev and Env proteins of simian immunodeficiency virus (SIV) are unique because upstream translation start codons are present that may modulate the expression of these viral proteins. This is true for the regular mRNAs, but we also report novel mRNA splicing variants that encode up to five upstream AUG (uAUG) codons. Their influence on Rev and Env translation was measured by mutational inactivation in reporter constructs and in the SIVmac239 strain. An intricate regulatory mechanism was disclosed that allows the virus to express a balanced amount of these two proteins. This insight also allows the design of vector constructs that efficiently express these proteins.",

keywords = "Alternative Splicing, Codon, Codon, Initiator, Gene Products, env/genetics, Gene Products, rev/genetics, Genes, Reporter, Genome, Viral, HEK293 Cells, Humans, Leukocytes, Mononuclear/virology, Models, Genetic, Mutation, Open Reading Frames, RNA Splicing, RNA, Messenger/metabolism, Sequence Analysis, DNA, Simian Immunodeficiency Virus/genetics",

author = "{van der Velden}, {Gisela J} and Bep Klaver and Das, {Atze T} and Ben Berkhout",

year = "2012",

month = nov,

doi = "10.1128/JVI.01532-12",

language = "English",

volume = "86",

pages = "12362--71",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "22",

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TY - JOUR

T1 - Upstream AUG codons in the simian immunodeficiency virus SIVmac239 genome regulate Rev and Env protein translation

AU - van der Velden, Gisela J

AU - Klaver, Bep

AU - Das, Atze T

AU - Berkhout, Ben

PY - 2012/11

Y1 - 2012/11

N2 - The mRNAs encoding the Rev and Env proteins of simian immunodeficiency virus (SIV) are unique because upstream translation start codons are present that may modulate the expression of these viral proteins. This is true for the regular mRNAs, but we also report novel mRNA splicing variants that encode up to five upstream AUG (uAUG) codons. Their influence on Rev and Env translation was measured by mutational inactivation in reporter constructs and in the SIVmac239 strain. An intricate regulatory mechanism was disclosed that allows the virus to express a balanced amount of these two proteins. This insight also allows the design of vector constructs that efficiently express these proteins.

AB - The mRNAs encoding the Rev and Env proteins of simian immunodeficiency virus (SIV) are unique because upstream translation start codons are present that may modulate the expression of these viral proteins. This is true for the regular mRNAs, but we also report novel mRNA splicing variants that encode up to five upstream AUG (uAUG) codons. Their influence on Rev and Env translation was measured by mutational inactivation in reporter constructs and in the SIVmac239 strain. An intricate regulatory mechanism was disclosed that allows the virus to express a balanced amount of these two proteins. This insight also allows the design of vector constructs that efficiently express these proteins.

KW - Alternative Splicing

KW - Codon

KW - Codon, Initiator

KW - Gene Products, env/genetics

KW - Gene Products, rev/genetics

KW - Genes, Reporter

KW - Genome, Viral

KW - HEK293 Cells

KW - Humans

KW - Leukocytes, Mononuclear/virology

KW - Models, Genetic

KW - Mutation

KW - Open Reading Frames

KW - RNA Splicing

KW - RNA, Messenger/metabolism

KW - Sequence Analysis, DNA

KW - Simian Immunodeficiency Virus/genetics

U2 - 10.1128/JVI.01532-12

DO - 10.1128/JVI.01532-12

M3 - Article

C2 - 22951834

SN - 0022-538X

VL - 86

SP - 12362

EP - 12371

JO - Journal of Virology

JF - Journal of Virology

IS - 22

ER -

Upstream AUG codons in the simian immunodeficiency virus SIVmac239 genome regulate Rev and Env protein translation

Abstract

Keywords

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