Ubiquitylation of the ER-Shaping Protein Lunapark via the CRL3KLHL12 Ubiquitin Ligase Complex

Laurensia Yuniati, Angela Lauriola, Manouk Gerritsen, Susana Abreu, Eric Ni, Chiara Tesoriero, Jacob O. Onireti, Teck Yew Low, Albert J.R. Heck, Andrea Vettori, Timothy Cardozo, Daniele Guardavaccaro*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Yuniati et al. perform a proteomic screen to identify E3 substrates that are ubiquitylated at cellular membranes and find that the ER-shaping protein Lunapark is ubiquitylated by the CRL3KLHL12 ubiquitin ligase. They show that Lunapark binds mTORC1 and affects its activity, and defects in Lunapark ubiquitylation lead to neurodevelopmental defects.

Original languageEnglish
Article number107664
Pages (from-to)1-20
Number of pages20
JournalCell Reports
Volume31
Issue number7
DOIs
Publication statusPublished - 19 May 2020

Keywords

  • cullin-RING ligases
  • endoplasmic reticulum
  • ER three-way junction
  • Lunapark
  • lysosome
  • mTORC1
  • ubiquitin

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