Two genes homologous with human protein S cDNA are located on chromosome 3

J. K. Ploos Van Amstel; A. L. Van Der Zanden; E. Bakker; P. H. Reitsma; R. M. Bertina

Two genes homologous with human protein S cDNA are located on chromosome 3

J. K. Ploos Van Amstel, A. L. Van Der Zanden, E. Bakker, P. H. Reitsma, R. M. Bertina

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3'-untranslated region, was isolated by a combination of antibody screening of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library. Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.

Original language	English
Pages (from-to)	982-987
Number of pages	6
Journal	Thrombosis and Haemostasis
Volume	58
Issue number	4
Publication status	Published - 1 Dec 1987
Externally published	Yes

Cite this

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title = "Two genes homologous with human protein S cDNA are located on chromosome 3",

abstract = "A cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3'-untranslated region, was isolated by a combination of antibody screening of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library. Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.",

author = "{Ploos Van Amstel}, {J. K.} and {Van Der Zanden}, {A. L.} and E. Bakker and Reitsma, {P. H.} and Bertina, {R. M.}",

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AU - Ploos Van Amstel, J. K.

AU - Van Der Zanden, A. L.

AU - Bakker, E.

AU - Reitsma, P. H.

AU - Bertina, R. M.

PY - 1987/12/1

Y1 - 1987/12/1

N2 - A cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3'-untranslated region, was isolated by a combination of antibody screening of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library. Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.

AB - A cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3'-untranslated region, was isolated by a combination of antibody screening of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library. Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.

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M3 - Article

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AN - SCOPUS:0023614852

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JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

IS - 4

ER -

Two genes homologous with human protein S cDNA are located on chromosome 3

Abstract

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