Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification

Kai Kretzschmar*, Kim E. Boonekamp, Margit Bleijs, Priyanca Asra, Mandy Koomen, Susana M. Chuva de Sousa Lopes, Barbara Giovannone, Hans Clevers

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    The skin epidermis is a highly compartmentalized tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific compartments in the skin but none of them is specific to the IFE. Here, we identify Troy as a marker of IFE and HF infundibulum basal layer cells in developing and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells, as well as their progeny, confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.

    Original languageEnglish
    Pages (from-to)2379-2394
    Number of pages16
    JournalStem Cell Reports
    Volume16
    Issue number9
    DOIs
    Publication statusPublished - 14 Sept 2021

    Keywords

    • epidermis
    • lineage tracing
    • organoids
    • single-cell transcriptomics
    • stem cells
    • Tnfrsf19
    • Troy

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