@article{000fa93d69e2433cbe36d78a696be0ad,
title = "Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification",
abstract = "The skin epidermis is a highly compartmentalized tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific compartments in the skin but none of them is specific to the IFE. Here, we identify Troy as a marker of IFE and HF infundibulum basal layer cells in developing and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells, as well as their progeny, confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.",
keywords = "epidermis, lineage tracing, organoids, single-cell transcriptomics, stem cells, Tnfrsf19, Troy",
author = "Kai Kretzschmar and Boonekamp, {Kim E.} and Margit Bleijs and Priyanca Asra and Mandy Koomen and {Chuva de Sousa Lopes}, {Susana M.} and Barbara Giovannone and Hans Clevers",
note = "Funding Information: We thank Onur Basak, Johan van Es and Maaike van den Born for experimental assistance; Stefan van der Elst and Reinier van den Linden for help with flow cytometry; the Utrecht Sequencing Facility (USEQ) for sequencing; Anna Alemany Arias for help with bioinformatics analysis and Anko de Graaff and the Hubrecht Imaging Centre (HIC) for assistance with microscopy. We thank the Gynaikon Clinic in Rotterdam for the efforts in providing the fetal material. This work was supported by a Sinergia grant from the Swiss National Science Foundation (SNSF/ CRSII3 160738-1 , to H.C.) and by a German Cancer Aid grant (via MSNZ W{\"u}rzburg, to K.K.). K.K. was recipient of a VENI grant from the Netherlands Organisation for Scientific Research (NWO-ZonMW, 016.166.140 ) and was a long-term fellow of the Human Frontier Science Program Organization (HFSPO, LT771/2015 ). Funding Information: We thank Onur Basak, Johan van Es and Maaike van den Born for experimental assistance; Stefan van der Elst and Reinier van den Linden for help with flow cytometry; the Utrecht Sequencing Facility (USEQ) for sequencing; Anna Alemany Arias for help with bioinformatics analysis and Anko de Graaff and the Hubrecht Imaging Centre (HIC) for assistance with microscopy. We thank the Gynaikon Clinic in Rotterdam for the efforts in providing the fetal material. This work was supported by a Sinergia grant from the Swiss National Science Foundation (SNSF/CRSII3 160738-1, to H.C.) and by a German Cancer Aid grant (via MSNZ W?rzburg, to K.K.). K.K. was recipient of a VENI grant from the Netherlands Organisation for Scientific Research (NWO-ZonMW, 016.166.140) and was a long-term fellow of the Human Frontier Science Program Organization (HFSPO, LT771/2015). Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = sep,
day = "14",
doi = "10.1016/j.stemcr.2021.07.007",
language = "English",
volume = "16",
pages = "2379--2394",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "9",
}