TY - JOUR
T1 - Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle
AU - Lewis, Fiona C.
AU - Cottle, Beverley J.
AU - Shone, Victoria
AU - Marazzi, Giovanna
AU - Sassoon, David
AU - Tseng, Cheyenne C.S.
AU - Dankers, Patricia Y.W.
AU - Chamuleau, Steven A.J.
AU - Nadal-Ginard, Bernardo
AU - Ellison-Hughes, Georgina M.
N1 - Funding Information:
This work was supported by the European Research Council, European Community 7th Framework project ENDOSTEM (contract number FP7-Health-2009-ENDOSTEM 241440 Activation of vasculature-associated stem cells and muscle stem cells for the repair and maintenance of muscle tissue). The authors have reported that they have no relationships relevant to the contents of this paper to disclose. Drs. Nadal-Ginard and Ellison-Hughes contributed equally to this work.
Publisher Copyright:
© 2017 The Authors
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Skeletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.
AB - Skeletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.
KW - allogeneic progenitor cells
KW - growth factors
KW - PICs
KW - porcine preclinical model
KW - regeneration
KW - skeletal muscle
UR - http://www.scopus.com/inward/record.url?scp=85040785558&partnerID=8YFLogxK
U2 - 10.1016/j.jacbts.2017.08.002
DO - 10.1016/j.jacbts.2017.08.002
M3 - Article
C2 - 30062184
AN - SCOPUS:85040785558
SN - 2452-302X
VL - 2
SP - 717
EP - 736
JO - JACC: Basic to Translational Science
JF - JACC: Basic to Translational Science
IS - 6
ER -