TY - JOUR
T1 - Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia
AU - Olde Loohuis, Loes M.
AU - Mangul, Serghei
AU - Ori, Anil P.S.
AU - Jospin, Guillaume
AU - Koslicki, David
AU - Yang, Harry Taegyun
AU - Wu, Timothy
AU - Boks, Marco P.
AU - Lomen-Hoerth, Catherine
AU - Wiedau-Pazos, Martina
AU - Cantor, Rita M.
AU - De Vos, Willem M.
AU - Kahn, René S.
AU - Eskin, Eleazar
AU - Ophoff, Roel A.
N1 - Funding Information:
This work is supported by NIH/NIMH R01 5R01MH090553 (BP samples), 5R01NS058980 (ALS samples) and R01MH078075 (SCZ, Control samples), R21MH098035 (replication sample) awarded to R.A.O. L.M.O.L was financially supported by the National Institute of Neurological Disorders And Stroke of the National Institutes of Health under Award Number T32NS048004. SM acknowledges support from a QCB Collaboratory Postdoctoral Fellowship, and the QCB Collaboratory community directed by Matteo Pellegrini. SM and EE are supported by National Science Foundation grants 0513612, 0731455, 0729049, 0916676, 1065276, 1302448, 1320589 and 1331176, and National Institutes of Health grants K25-HL080079, U01-DA024417, P01-HL30568, P01-HL28481, R01-GM083198, R01-ES021801, R01-MH101782 and R01-ES022282. The authors thank Dr. Jonathan Eisen for helpful discussions and insights throughout the course of this project. We thank Dr. Lana Martin for helpful edits to the manuscript. Finally, we thank the study subjects for their participation.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The role of the human microbiome in health and disease is increasingly appreciated. We studied the composition of microbial communities present in blood across 192 individuals, including healthy controls and patients with three disorders affecting the brain: schizophrenia, amyotrophic lateral sclerosis, and bipolar disorder. By using high-quality unmapped RNA sequencing reads as candidate microbial reads, we performed profiling of microbial transcripts detected in whole blood. We were able to detect a wide range of bacterial and archaeal phyla in blood. Interestingly, we observed an increased microbial diversity in schizophrenia patients compared to the three other groups. We replicated this finding in an independent schizophrenia case-control cohort. This increased diversity is inversely correlated with estimated cell abundance of a subpopulation of CD8+ memory T cells in healthy controls, supporting a link between microbial products found in blood, immunity and schizophrenia.
AB - The role of the human microbiome in health and disease is increasingly appreciated. We studied the composition of microbial communities present in blood across 192 individuals, including healthy controls and patients with three disorders affecting the brain: schizophrenia, amyotrophic lateral sclerosis, and bipolar disorder. By using high-quality unmapped RNA sequencing reads as candidate microbial reads, we performed profiling of microbial transcripts detected in whole blood. We were able to detect a wide range of bacterial and archaeal phyla in blood. Interestingly, we observed an increased microbial diversity in schizophrenia patients compared to the three other groups. We replicated this finding in an independent schizophrenia case-control cohort. This increased diversity is inversely correlated with estimated cell abundance of a subpopulation of CD8+ memory T cells in healthy controls, supporting a link between microbial products found in blood, immunity and schizophrenia.
UR - http://www.scopus.com/inward/record.url?scp=85046870937&partnerID=8YFLogxK
U2 - 10.1038/s41398-018-0107-9
DO - 10.1038/s41398-018-0107-9
M3 - Article
AN - SCOPUS:85046870937
SN - 2158-3188
VL - 8
JO - Translational Psychiatry
JF - Translational Psychiatry
IS - 1
M1 - 96
ER -