TY - JOUR
T1 - Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano
AU - Grudniewska, Magda
AU - Mouton, Stijn
AU - Simanov, Daniil
AU - Beltman, Frank
AU - Grelling, Margriet
AU - De Mulder, Katrien
AU - Arindrarto, Wibowo
AU - Weissert, Philipp M.
AU - van der Elst, Stefan
AU - Berezikov, Eugene
PY - 2016/12/20
Y1 - 2016/12/20
N2 - The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.
AB - The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.
UR - http://www.scopus.com/inward/record.url?scp=85006942226&partnerID=8YFLogxK
U2 - 10.7554/eLife.20607
DO - 10.7554/eLife.20607
M3 - Article
C2 - 27997336
AN - SCOPUS:85006942226
SN - 2050-084X
VL - 5
JO - eLife
JF - eLife
M1 - e20607
ER -