Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study

Srikant Ambatipudi; Cyrille Cuenin; Hector Hernandez-Vargas; Akram Ghantous; Florence Le Calvez-Kelm; Rudolf Kaaks; Myrto Barrdahl; Heiner Boeing; Krasimira Aleksandrova; Antonia Trichopoulou; Pagona Lagiou; Androniki Naska; Domenico Palli; Vittorio Krogh; Silvia Polidoro; Rosario Tumino; Salvatore Panico; Bas Bueno-De-Mesquita; Petra HM Peeters; José Ramón Quirós; Carmen Navarro; Eva Ardanaz; Miren Dorronsoro; Tim Key; Paolo Vineis; Neil Murphy; Elio Riboli; Isabelle Romieu; Zdenko Herceg

doi:10.2217/epi-2016-0001

Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study

Srikant Ambatipudi, Cyrille Cuenin, Hector Hernandez-Vargas, Akram Ghantous, Florence Le Calvez-Kelm, Rudolf Kaaks, Myrto Barrdahl, Heiner Boeing, Krasimira Aleksandrova, Antonia Trichopoulou, Pagona Lagiou, Androniki Naska, Domenico Palli, Vittorio Krogh, Silvia Polidoro, Rosario Tumino, Salvatore Panico, Bas Bueno-De-Mesquita, Petra HM Peeters, José Ramón QuirósCarmen Navarro, Eva Ardanaz, Miren Dorronsoro, Tim Key, Paolo Vineis, Neil Murphy, Elio Riboli, Isabelle Romieu, Zdenko Herceg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Aim: Epigenetic changes may occur in response to environmental stressors, and an altered epigenome pattern may represent a stable signature of environmental exposure. Materials & methods: Here, we examined the potential of DNA methylation changes in 910 prediagnostic peripheral blood samples as a marker of exposure to tobacco smoke in a large multinational cohort. Results: We identified 748 CpG sites that were differentially methylated between smokers and nonsmokers, among which we identified novel regionally clustered CpGs associated with active smoking. Importantly, we found a marked reversibility of methylation changes after smoking cessation, although specific genes remained differentially methylated up to 22 years after cessation. Conclusion: Our study has comprehensively cataloged the smoking-associated DNA methylation alterations and showed that these alterations are reversible after smoking cessation.

Original language	English
Pages (from-to)	599-618
Number of pages	20
Journal	Epigenomics
Volume	8
Issue number	5
DOIs	https://doi.org/10.2217/epi-2016-0001
Publication status	Published - 1 May 2016

Keywords

DNA methylome
epigenetic signature
prospective cohort
tobacco smoking

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Ambatipudi, S., Cuenin, C., Hernandez-Vargas, H., Ghantous, A., Le Calvez-Kelm, F., Kaaks, R., Barrdahl, M., Boeing, H., Aleksandrova, K., Trichopoulou, A., Lagiou, P., Naska, A., Palli, D., Krogh, V., Polidoro, S., Tumino, R., Panico, S., Bueno-De-Mesquita, B., Peeters, P. HM., ... Herceg, Z. (2016). Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study. Epigenomics, 8(5), 599-618. https://doi.org/10.2217/epi-2016-0001

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title = "Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study",

abstract = "Aim: Epigenetic changes may occur in response to environmental stressors, and an altered epigenome pattern may represent a stable signature of environmental exposure. Materials & methods: Here, we examined the potential of DNA methylation changes in 910 prediagnostic peripheral blood samples as a marker of exposure to tobacco smoke in a large multinational cohort. Results: We identified 748 CpG sites that were differentially methylated between smokers and nonsmokers, among which we identified novel regionally clustered CpGs associated with active smoking. Importantly, we found a marked reversibility of methylation changes after smoking cessation, although specific genes remained differentially methylated up to 22 years after cessation. Conclusion: Our study has comprehensively cataloged the smoking-associated DNA methylation alterations and showed that these alterations are reversible after smoking cessation.",

keywords = "DNA methylome, epigenetic signature, prospective cohort, tobacco smoking",

author = "Srikant Ambatipudi and Cyrille Cuenin and Hector Hernandez-Vargas and Akram Ghantous and {Le Calvez-Kelm}, Florence and Rudolf Kaaks and Myrto Barrdahl and Heiner Boeing and Krasimira Aleksandrova and Antonia Trichopoulou and Pagona Lagiou and Androniki Naska and Domenico Palli and Vittorio Krogh and Silvia Polidoro and Rosario Tumino and Salvatore Panico and Bas Bueno-De-Mesquita and Peeters, {Petra HM} and Quir{\'o}s, {Jos{\'e} Ram{\'o}n} and Carmen Navarro and Eva Ardanaz and Miren Dorronsoro and Tim Key and Paolo Vineis and Neil Murphy and Elio Riboli and Isabelle Romieu and Zdenko Herceg",

year = "2016",

month = may,

day = "1",

doi = "10.2217/epi-2016-0001",

language = "English",

volume = "8",

pages = "599--618",

journal = "Epigenomics",

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Ambatipudi, S, Cuenin, C, Hernandez-Vargas, H, Ghantous, A, Le Calvez-Kelm, F, Kaaks, R, Barrdahl, M, Boeing, H, Aleksandrova, K, Trichopoulou, A, Lagiou, P, Naska, A, Palli, D, Krogh, V, Polidoro, S, Tumino, R, Panico, S, Bueno-De-Mesquita, B, Peeters, PHM, Quirós, JR, Navarro, C, Ardanaz, E, Dorronsoro, M, Key, T, Vineis, P, Murphy, N, Riboli, E, Romieu, I & Herceg, Z 2016, 'Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study', Epigenomics, vol. 8, no. 5, pp. 599-618. https://doi.org/10.2217/epi-2016-0001

TY - JOUR

T1 - Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study

AU - Ambatipudi, Srikant

AU - Cuenin, Cyrille

AU - Hernandez-Vargas, Hector

AU - Ghantous, Akram

AU - Le Calvez-Kelm, Florence

AU - Kaaks, Rudolf

AU - Barrdahl, Myrto

AU - Boeing, Heiner

AU - Aleksandrova, Krasimira

AU - Trichopoulou, Antonia

AU - Lagiou, Pagona

AU - Naska, Androniki

AU - Palli, Domenico

AU - Krogh, Vittorio

AU - Polidoro, Silvia

AU - Tumino, Rosario

AU - Panico, Salvatore

AU - Bueno-De-Mesquita, Bas

AU - Peeters, Petra HM

AU - Quirós, José Ramón

AU - Navarro, Carmen

AU - Ardanaz, Eva

AU - Dorronsoro, Miren

AU - Key, Tim

AU - Vineis, Paolo

AU - Murphy, Neil

AU - Riboli, Elio

AU - Romieu, Isabelle

AU - Herceg, Zdenko

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Aim: Epigenetic changes may occur in response to environmental stressors, and an altered epigenome pattern may represent a stable signature of environmental exposure. Materials & methods: Here, we examined the potential of DNA methylation changes in 910 prediagnostic peripheral blood samples as a marker of exposure to tobacco smoke in a large multinational cohort. Results: We identified 748 CpG sites that were differentially methylated between smokers and nonsmokers, among which we identified novel regionally clustered CpGs associated with active smoking. Importantly, we found a marked reversibility of methylation changes after smoking cessation, although specific genes remained differentially methylated up to 22 years after cessation. Conclusion: Our study has comprehensively cataloged the smoking-associated DNA methylation alterations and showed that these alterations are reversible after smoking cessation.

AB - Aim: Epigenetic changes may occur in response to environmental stressors, and an altered epigenome pattern may represent a stable signature of environmental exposure. Materials & methods: Here, we examined the potential of DNA methylation changes in 910 prediagnostic peripheral blood samples as a marker of exposure to tobacco smoke in a large multinational cohort. Results: We identified 748 CpG sites that were differentially methylated between smokers and nonsmokers, among which we identified novel regionally clustered CpGs associated with active smoking. Importantly, we found a marked reversibility of methylation changes after smoking cessation, although specific genes remained differentially methylated up to 22 years after cessation. Conclusion: Our study has comprehensively cataloged the smoking-associated DNA methylation alterations and showed that these alterations are reversible after smoking cessation.

KW - DNA methylome

KW - epigenetic signature

KW - prospective cohort

KW - tobacco smoking

UR - http://www.scopus.com/inward/record.url?scp=84969931919&partnerID=8YFLogxK

U2 - 10.2217/epi-2016-0001

DO - 10.2217/epi-2016-0001

M3 - Article

C2 - 26864933

AN - SCOPUS:84969931919

SN - 1750-1911

VL - 8

SP - 599

EP - 618

JO - Epigenomics

JF - Epigenomics

IS - 5

ER -

Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study

Abstract

Keywords

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