@article{1530eb2c3151445b9382dad35884716c,
title = "Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition",
abstract = "Accurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through regulated protein degradation. Here, we show that widespread and temporally controlled mRNA decay acts as an additional mechanism for gene expression regulation during the cell cycle in human cells. We find that two waves of mRNA decay occur sequentially during the mitosis-to-G1 phase transition, and we identify the deadenylase CNOT1 as a factor that contributes to mRNA decay during this cell cycle transition. Collectively, our data show that, akin to protein degradation, scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase.",
keywords = "Cell Cycle/genetics, Cell Line, Gene Expression Regulation, HEK293 Cells, Humans, RNA Stability/physiology, Sequence Analysis, RNA, Transcription Factors/metabolism, Human, single-cell sequencing, cell cycle, gene regulation, mRNA decay",
author = "Lenno Krenning and Stijn Sonneveld and Marvin Tanenbaum",
note = "Funding Information: We thank members of the Tanenbaum group for helpful discussions, and Xiaowei Yan for help during the initial stages of the project. We thank the Hubrecht Institute flow cytometry facility and single-cell sequencing facility (now Single Cell Discoveries) for their technical support. This work was financially supported by the European Research Council (ERC) through an ERC starting grant (ERCSTG 677936-RNAREG) to MET; MET is also supported by the Oncode Institute that is partially funded by the Dutch Cancer Society (KWF). Funding Information: We thank members of the Tanenbaum group for helpful discussions, and Xiaowei Yan for help during the initial stages of the project. We thank the Hubrecht Institute flow cytometry facility and single-cell sequencing facility (now Single Cell Discoveries) for their technical support. This work was financially supported by the European Research Council (ERC) through an ERC starting grant (ERCSTG 677936-RNAREG) to MET; MET is also supported by the Oncode Institute that is partially funded by the Dutch Cancer Society (KWF). Funder Grant reference number Author European Research Council ERCSTG 677936-RNAREG Marvin Tanenbaum The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} Krenning et al.",
year = "2022",
month = feb,
doi = "10.7554/ELIFE.71356",
language = "English",
volume = "11",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}