Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

Fanny Lepiemme; Carla G Silva; Laurent Nguyen

doi:10.1016/j.xpro.2021.100467

Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

Fanny Lepiemme, Carla G Silva, Laurent Nguyen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement. For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).

Original language	English
Article number	100467
Journal	STAR protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100467
Publication status	Published - 2021
Externally published	Yes

Keywords

Cell Biology
Microbiology
Neuroscience

Access to Document

10.1016/j.xpro.2021.100467Licence: CC BY-NC-ND

Cite this

@article{88f18e61279745e89c18e066b2e03bbf,

title = "Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants",

abstract = "Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement. For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).",

keywords = "Cell Biology, Microbiology, Neuroscience",

author = "Fanny Lepiemme and Silva, {Carla G} and Laurent Nguyen",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

doi = "10.1016/j.xpro.2021.100467",

language = "English",

volume = "2",

number = "2",

}

TY - JOUR

T1 - Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

AU - Lepiemme, Fanny

AU - Silva, Carla G

AU - Nguyen, Laurent

PY - 2021

Y1 - 2021

N2 - Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement. For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).

AB - Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement. For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).

KW - Cell Biology

KW - Microbiology

KW - Neuroscience

UR - http://www.scopus.com/inward/record.url?scp=85104280043&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2021.100467

DO - 10.1016/j.xpro.2021.100467

M3 - Article

SN - 2666-1667

VL - 2

JO - STAR protocols

JF - STAR protocols

IS - 2

M1 - 100467

ER -

Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this