TY - JOUR
T1 - The Value of Single-cell Technologies in Solid Organ Transplantation Studies
AU - Peereboom, Emma T M
AU - Matern, Benedict M
AU - Spierings, Eric
AU - Geneugelijk, Kirsten
N1 - Funding Information:
This study was supported by research funding from the Dutch Kidney Foundation project code CP1801 “Prediction of Kidney Graft Survival by Immune Profiling - Towards Clinical Application in Personalized Medicine,” by research funding from EU Horizon 2020 for the ULISES project, code 899708, and by research funding from the International HLA and Immunogenetics Workshop Foundation.
Publisher Copyright:
© 2022 Lippincott Williams and Wilkins. All rights reserved.
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Single-cell technologies open up new opportunities to explore the behavior of cells at the individual level. For solid organ transplantation, single-cell technologies can provide in-depth insights into the underlying mechanisms of the immunological processes involved in alloimmune responses after transplantation by investigating the role of individual cells in tolerance and rejection. Here, we review the value of single-cell technologies, including cytometry by time-of-flight and single-cell RNA sequencing, in the context of solid organ transplantation research. Various applications of single-cell technologies are addressed, such as the characterization and identification of immune cell subsets involved in rejection or tolerance. In addition, we explore the opportunities for analyzing specific alloreactive T- or B-cell clones by linking phenotype data to T- or B-cell receptor data, and for distinguishing donor- from recipient-derived immune cells. Moreover, we discuss the use of single-cell technologies in biomarker identification and risk stratification, as well as the remaining challenges. Together, this review highlights that single-cell approaches contribute to a better understanding of underlying immunological mechanisms of rejection and tolerance, thereby potentially accelerating the development of new or improved therapies to avoid allograft rejection.
AB - Single-cell technologies open up new opportunities to explore the behavior of cells at the individual level. For solid organ transplantation, single-cell technologies can provide in-depth insights into the underlying mechanisms of the immunological processes involved in alloimmune responses after transplantation by investigating the role of individual cells in tolerance and rejection. Here, we review the value of single-cell technologies, including cytometry by time-of-flight and single-cell RNA sequencing, in the context of solid organ transplantation research. Various applications of single-cell technologies are addressed, such as the characterization and identification of immune cell subsets involved in rejection or tolerance. In addition, we explore the opportunities for analyzing specific alloreactive T- or B-cell clones by linking phenotype data to T- or B-cell receptor data, and for distinguishing donor- from recipient-derived immune cells. Moreover, we discuss the use of single-cell technologies in biomarker identification and risk stratification, as well as the remaining challenges. Together, this review highlights that single-cell approaches contribute to a better understanding of underlying immunological mechanisms of rejection and tolerance, thereby potentially accelerating the development of new or improved therapies to avoid allograft rejection.
KW - Graft Rejection
KW - Histocompatibility
KW - Immune Tolerance
KW - Organ Transplantation/adverse effects
KW - Transplantation, Homologous
UR - http://www.scopus.com/inward/record.url?scp=85142940414&partnerID=8YFLogxK
U2 - 10.1097/TP.0000000000004237
DO - 10.1097/TP.0000000000004237
M3 - Review article
C2 - 35876376
SN - 0041-1337
VL - 106
SP - 2325
EP - 2337
JO - Transplantation
JF - Transplantation
IS - 12
ER -