The utility of fluorescent in vivo reporter genes in molecular cardiology

P A Doevendans; K D Becker; R H An; R S Kass

doi:10.1006/bbrc.1996.0747

The utility of fluorescent in vivo reporter genes in molecular cardiology

P A Doevendans, K D Becker, R H An, R S Kass

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In vivo reporter genes can be used in different ways in molecular cardiology. In this paper studies are presented using the green fluorescent protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identification prior to patch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells.

Original language	English
Pages (from-to)	352-8
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	222
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1996.0747
Publication status	Published - 1996

Keywords

Animals
Animals, Newborn
Calcium Channels
Calcium Channels, L-Type
Cell Line
Cells, Cultured
Cercopithecus aethiops
Cloning, Molecular
Fibroblasts
Green Fluorescent Proteins
Heart Ventricles
Humans
Kidney
Luminescent Proteins
Mice
Myosin Light Chains
Patch-Clamp Techniques
Plasmids
Point Mutation
Promoter Regions, Genetic
Recombinant Proteins
Restriction Mapping
Sensitivity and Specificity
Transfection

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10.1006/bbrc.1996.0747

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title = "The utility of fluorescent in vivo reporter genes in molecular cardiology",

abstract = "In vivo reporter genes can be used in different ways in molecular cardiology. In this paper studies are presented using the green fluorescent protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identification prior to patch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells.",

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author = "Doevendans, {P A} and Becker, {K D} and An, {R H} and Kass, {R S}",

year = "1996",

doi = "10.1006/bbrc.1996.0747",

language = "English",

volume = "222",

pages = "352--8",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - The utility of fluorescent in vivo reporter genes in molecular cardiology

AU - Doevendans, P A

AU - Becker, K D

AU - An, R H

AU - Kass, R S

PY - 1996

Y1 - 1996

N2 - In vivo reporter genes can be used in different ways in molecular cardiology. In this paper studies are presented using the green fluorescent protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identification prior to patch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells.

AB - In vivo reporter genes can be used in different ways in molecular cardiology. In this paper studies are presented using the green fluorescent protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identification prior to patch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells.

KW - Animals

KW - Animals, Newborn

KW - Calcium Channels

KW - Calcium Channels, L-Type

KW - Cell Line

KW - Cells, Cultured

KW - Cercopithecus aethiops

KW - Cloning, Molecular

KW - Fibroblasts

KW - Green Fluorescent Proteins

KW - Heart Ventricles

KW - Humans

KW - Kidney

KW - Luminescent Proteins

KW - Mice

KW - Myosin Light Chains

KW - Patch-Clamp Techniques

KW - Plasmids

KW - Point Mutation

KW - Promoter Regions, Genetic

KW - Recombinant Proteins

KW - Restriction Mapping

KW - Sensitivity and Specificity

KW - Transfection

U2 - 10.1006/bbrc.1996.0747

DO - 10.1006/bbrc.1996.0747

M3 - Article

C2 - 8670208

SN - 0006-291X

VL - 222

SP - 352

EP - 358

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

The utility of fluorescent in vivo reporter genes in molecular cardiology

Abstract

Keywords

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