TY - JOUR
T1 - The role of HSV-induced Fc- and C3b(i)-receptors in bacterial adherence
AU - De Graaf-Miltenburg, L. A.M.
AU - Van Vliet, K. E.
AU - Ten Hagen, T. L.M.
AU - Verhoef, J.
AU - Van Strijp, J. A.G.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fluorescent quantitation of FITC-labelled bacteria, was developed. Opsonisation of Staphylococcus epidermidis with human serum, resulted in a marked increase in adherence to HSV-infected cells and revealed a role for C3b(i)R- and FcR-mediated adhesion. However, the enhanced adherence never exceeded the level of attachment to non-infected cells. Increased adherence of other pathogenic bacteria, including Escherichia coli, Streptococcus pneumoniae, Haemophilus influenzae and Pseudomonas aeruginosa was not observed, indicating that the HSV-receptors play a minor role in secondary infections. Bacterial adhesion factors such as the fimbriae of E. coli played a more dominant role in the adherence of bacteria to HSV-infected cells.
AB - Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fluorescent quantitation of FITC-labelled bacteria, was developed. Opsonisation of Staphylococcus epidermidis with human serum, resulted in a marked increase in adherence to HSV-infected cells and revealed a role for C3b(i)R- and FcR-mediated adhesion. However, the enhanced adherence never exceeded the level of attachment to non-infected cells. Increased adherence of other pathogenic bacteria, including Escherichia coli, Streptococcus pneumoniae, Haemophilus influenzae and Pseudomonas aeruginosa was not observed, indicating that the HSV-receptors play a minor role in secondary infections. Bacterial adhesion factors such as the fimbriae of E. coli played a more dominant role in the adherence of bacteria to HSV-infected cells.
UR - http://www.scopus.com/inward/record.url?scp=0028009784&partnerID=8YFLogxK
U2 - 10.1099/00222615-40-1-48
DO - 10.1099/00222615-40-1-48
M3 - Article
C2 - 7904650
AN - SCOPUS:0028009784
SN - 0022-2615
VL - 40
SP - 48
EP - 54
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 1
ER -