The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice

Patrick M Meyer Sauteur; Ruben C A de Groot; Silvia C Estevão; Theo Hoogenboezem; Adrianus C J M de Bruijn; Marcel Sluijter; Marjolein J W de Bruijn; Ismé M De Kleer; Rien van Haperen; Judith M A van den Brand; Debby Bogaert; Pieter L. A. Fraaij; Cornelis Vink; Rudi W. Hendriks; Janneke N. Samsom; Wendy W J Unger; Annemarie M. C. van Rossum

doi:10.1093/infdis/jix559

The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice

Patrick M Meyer Sauteur, Ruben C A de Groot, Silvia C Estevão, Theo Hoogenboezem, Adrianus C J M de Bruijn, Marcel Sluijter, Marjolein J W de Bruijn, Ismé M De Kleer, Rien van Haperen, Judith M A van den Brand, Debby Bogaert, Pieter L. A. Fraaij, Cornelis Vink, Rudi W. Hendriks, Janneke N. Samsom, Wendy W J Unger, Annemarie M. C. van Rossum

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background. Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. Methods. In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Results. Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose-A nd lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient ìMT mice, whereas this enabled ìMT mice to clear pulmonary Mp infection. Conclusions. We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

Original language	English
Pages (from-to)	298-309
Number of pages	12
Journal	Journal of Infectious Diseases
Volume	217
Issue number	2
Early online date	1 Nov 2017
DOIs	https://doi.org/10.1093/infdis/jix559
Publication status	Published - 15 Jan 2018

Keywords

Journal Article
carriage
mouse model
pneumonia
Mycoplasma pneumoniae
B cells
Immunoglobulin G/blood
Antibodies, Bacterial/blood
Mice, Inbred C57BL
Nasal Mucosa/immunology
Pneumonia, Mycoplasma/immunology
Mycoplasma pneumoniae/immunology
B-Lymphocytes/immunology
Animals
Carrier State/immunology
Immunoglobulin A/analysis
Nasopharynx/immunology

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10.1093/infdis/jix559

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Meyer Sauteur, P. M., de Groot, R. C. A., Estevão, S. C., Hoogenboezem, T., de Bruijn, A. C. J. M., Sluijter, M., de Bruijn, M. J. W., De Kleer, I. M., van Haperen, R., van den Brand, J. M. A., Bogaert, D., Fraaij, P. L. A., Vink, C., Hendriks, R. W., Samsom, J. N., Unger, W. W. J., & van Rossum, A. M. C. (2018). The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice. Journal of Infectious Diseases, 217(2), 298-309. https://doi.org/10.1093/infdis/jix559

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title = "The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice",

abstract = "Background. Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. Methods. In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Results. Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose-A nd lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient {\`i}MT mice, whereas this enabled {\`i}MT mice to clear pulmonary Mp infection. Conclusions. We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.",

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author = "{Meyer Sauteur}, {Patrick M} and {de Groot}, {Ruben C A} and Estev{\~a}o, {Silvia C} and Theo Hoogenboezem and {de Bruijn}, {Adrianus C J M} and Marcel Sluijter and {de Bruijn}, {Marjolein J W} and {De Kleer}, {Ism{\'e} M} and {van Haperen}, Rien and {van den Brand}, {Judith M A} and Debby Bogaert and Fraaij, {Pieter L. A.} and Cornelis Vink and Hendriks, {Rudi W.} and Samsom, {Janneke N.} and Unger, {Wendy W J} and {van Rossum}, {Annemarie M. C.}",

year = "2018",

month = jan,

day = "15",

doi = "10.1093/infdis/jix559",

language = "English",

volume = "217",

pages = "298--309",

journal = "Journal of Infectious Diseases",

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Meyer Sauteur, PM, de Groot, RCA, Estevão, SC, Hoogenboezem, T, de Bruijn, ACJM, Sluijter, M, de Bruijn, MJW, De Kleer, IM, van Haperen, R, van den Brand, JMA, Bogaert, D, Fraaij, PLA, Vink, C, Hendriks, RW, Samsom, JN, Unger, WWJ & van Rossum, AMC 2018, 'The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice', Journal of Infectious Diseases, vol. 217, no. 2, pp. 298-309. https://doi.org/10.1093/infdis/jix559

TY - JOUR

T1 - The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice

AU - Meyer Sauteur, Patrick M

AU - de Groot, Ruben C A

AU - Estevão, Silvia C

AU - Hoogenboezem, Theo

AU - de Bruijn, Adrianus C J M

AU - Sluijter, Marcel

AU - de Bruijn, Marjolein J W

AU - De Kleer, Ismé M

AU - van Haperen, Rien

AU - van den Brand, Judith M A

AU - Bogaert, Debby

AU - Fraaij, Pieter L. A.

AU - Vink, Cornelis

AU - Hendriks, Rudi W.

AU - Samsom, Janneke N.

AU - Unger, Wendy W J

AU - van Rossum, Annemarie M. C.

PY - 2018/1/15

Y1 - 2018/1/15

N2 - Background. Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. Methods. In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Results. Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose-A nd lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient ìMT mice, whereas this enabled ìMT mice to clear pulmonary Mp infection. Conclusions. We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

AB - Background. Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. Methods. In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Results. Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose-A nd lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient ìMT mice, whereas this enabled ìMT mice to clear pulmonary Mp infection. Conclusions. We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

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KW - Mycoplasma pneumoniae

KW - B cells

KW - Immunoglobulin G/blood

KW - Antibodies, Bacterial/blood

KW - Mice, Inbred C57BL

KW - Nasal Mucosa/immunology

KW - Pneumonia, Mycoplasma/immunology

KW - Mycoplasma pneumoniae/immunology

KW - B-Lymphocytes/immunology

KW - Animals

KW - Carrier State/immunology

KW - Immunoglobulin A/analysis

KW - Nasopharynx/immunology

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DO - 10.1093/infdis/jix559

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SN - 0022-1899

VL - 217

SP - 298

EP - 309

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 2

ER -

The role of B cells in carriage and clearance of Mycoplasma pneumoniae from the respiratory tract of mice

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