The microRNA-producing enzyme Dicer1 is essential for zebrafish development

Erno Wienholds; Marco J. Koudijs; Freek J M Van Eeden; Edwin Cuppen; Ronald H A Plasterk

doi:10.1038/ng1251

The microRNA-producing enzyme Dicer1 is essential for zebrafish development

Erno Wienholds
, Marco J. Koudijs
, Freek J M Van Eeden
, Edwin Cuppen
, Ronald H A Plasterk^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

263 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

Original language	English
Pages (from-to)	217-218
Number of pages	2
Journal	Nature Genetics
Volume	35
Issue number	3
DOIs	https://doi.org/10.1038/ng1251
Publication status	Published - 1 Nov 2003

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10.1038/ng1251

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abstract = "MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.",

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AU - Wienholds, Erno

AU - Koudijs, Marco J.

AU - Van Eeden, Freek J M

AU - Cuppen, Edwin

AU - Plasterk, Ronald H A

PY - 2003/11/1

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N2 - MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

AB - MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

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The microRNA-producing enzyme Dicer1 is essential for zebrafish development

Abstract

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