TY - JOUR
T1 - The human protein S locus
T2 - Identification of the PSα gene as a site of liver protein S messenger RNA synthesis
AU - Ploos van Amstel, Hans K.
AU - Reitsma, Pieter H.
AU - Bertina, Rogier M.
PY - 1988/12/30
Y1 - 1988/12/30
N2 - The protein S locus, situated on chromosome 3, consists of two protein S genes. Here, we report the cloning and complete nucleotide sequence of the 3′-untranslated region of the two genes designated PSα and PSβ. Both regions span approximately 1,200 nucleotides. They show a high degree (-97%) of homology, with deviations caused by small deletions, insertions and point mutations. Comparison of PSα and PSβ with the reported protein S liver cDNAs, shows that the latter all originate from the PSα gene. The PSα gene therefore is marked as the major site of synthesis of liver protein S mRNA. Sequence comparison with the bovine protein S cDNA reveals that the PSβ gene has accumulated a few more mutations than the PSα gene since duplication of the ancestral protein S gene that seems to have occurred recently during primate evolution.
AB - The protein S locus, situated on chromosome 3, consists of two protein S genes. Here, we report the cloning and complete nucleotide sequence of the 3′-untranslated region of the two genes designated PSα and PSβ. Both regions span approximately 1,200 nucleotides. They show a high degree (-97%) of homology, with deviations caused by small deletions, insertions and point mutations. Comparison of PSα and PSβ with the reported protein S liver cDNAs, shows that the latter all originate from the PSα gene. The PSα gene therefore is marked as the major site of synthesis of liver protein S mRNA. Sequence comparison with the bovine protein S cDNA reveals that the PSβ gene has accumulated a few more mutations than the PSα gene since duplication of the ancestral protein S gene that seems to have occurred recently during primate evolution.
UR - http://www.scopus.com/inward/record.url?scp=0024267310&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)80978-1
DO - 10.1016/S0006-291X(88)80978-1
M3 - Article
C2 - 2974696
AN - SCOPUS:0024267310
SN - 0006-291X
VL - 157
SP - 1033
EP - 1038
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -