TY - JOUR
T1 - The function of GORASPs in Golgi apparatus organization in vivo
AU - Grond, Rianne
AU - Veenendaal, Tineke
AU - Duran, Juan M.
AU - Raote, Ishier
AU - van Es, Johan H.
AU - Corstjens, Sebastiaan
AU - Delfgou, Laura
AU - El Haddouti, Benaissa
AU - Malhotra, Vivek
AU - Rabouille, Catherine
N1 - Funding Information:
V. Malhotra is an Institució Catalana de Recerca i Estudis Avançats professor at the Centre for Genomic Regulation, and work in his laboratory is funded by the Ministerio de Economía y Competitividad (grants SEV-2012-0208, BFU2013-44188-P, and CSD2009-00016). I. Raote acknowledges funding from the Spanish Ministry of Science and Innovation (IJCI-2017-34751).
Publisher Copyright:
© 2020 Grond et al.
PY - 2020/9/7
Y1 - 2020/9/7
N2 - In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.
AB - In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.
UR - http://www.scopus.com/inward/record.url?scp=85086930138&partnerID=8YFLogxK
U2 - 10.1083/jcb.202004191
DO - 10.1083/jcb.202004191
M3 - Article
C2 - 32573693
AN - SCOPUS:85086930138
SN - 1540-8140
VL - 219
JO - The Journal of cell biology
JF - The Journal of cell biology
IS - 9
M1 - e202004191
ER -