The Drosophila GMII gene encodes a Golgi α-mannosidase II

Catherine Rabouille; Douglas A. Kuntz; Anne Lockyer; Rose Watson; Tara Signorelli; David R. Rose; Marcel Van Den Heuvel; David B. Roberts

The Drosophila GMII gene encodes a Golgi α-mannosidase II

Catherine Rabouille^*, Douglas A. Kuntz, Anne Lockyer, Rose Watson, Tara Signorelli, David R. Rose, Marcel Van Den Heuvel, David B. Roberts

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

77 Citations (Scopus)

Abstract

In this paper we show the organisation of the Drosophila gene encoding a Golgi α-mannosidase II. We demonstrate that it encodes a functional homologue of the mouse Golgi α-mannosidase II. The Drosophila and mouse cDNA sequences translate into amino acid sequences which show 41% identity and 61% similarity. Expression of the Drosophila GMII sequence in CHOP cells produces an enzyme which has mannosidase activity and is inhibited by swainsonine and by CuSO₄. In cultured Drosophila cells and in Drosophila embryos, antibodies raised against a C-terminal peptide localise this product mainly to the Golgi apparatus as identified by cryo-immuno electron microscopy studies and by antibodies raised against known mammalian Golgi proteins. We discuss these results in terms of the possible use of dGMII as a Drosophila Golgi marker.

Original language	English
Pages (from-to)	3319-3330
Number of pages	12
Journal	Journal of Cell Science
Volume	112
Issue number	19
Publication status	Published - 10 Nov 1999

Keywords

α-mannosidase II
Cryo-immuno electron microscopy
Drosophila embryos
Drosophila S2 cell
Genomic sequence
Glycosyl transferase
Golgi apparatus
Indirect immunofluorescence
N-glycosylation pathway

Cite this

@article{2758fe7dbb244889819f483049504686,

title = "The Drosophila GMII gene encodes a Golgi α-mannosidase II",

abstract = "In this paper we show the organisation of the Drosophila gene encoding a Golgi α-mannosidase II. We demonstrate that it encodes a functional homologue of the mouse Golgi α-mannosidase II. The Drosophila and mouse cDNA sequences translate into amino acid sequences which show 41% identity and 61% similarity. Expression of the Drosophila GMII sequence in CHOP cells produces an enzyme which has mannosidase activity and is inhibited by swainsonine and by CuSO4. In cultured Drosophila cells and in Drosophila embryos, antibodies raised against a C-terminal peptide localise this product mainly to the Golgi apparatus as identified by cryo-immuno electron microscopy studies and by antibodies raised against known mammalian Golgi proteins. We discuss these results in terms of the possible use of dGMII as a Drosophila Golgi marker.",

keywords = "α-mannosidase II, Cryo-immuno electron microscopy, Drosophila embryos, Drosophila S2 cell, Genomic sequence, Glycosyl transferase, Golgi apparatus, Indirect immunofluorescence, N-glycosylation pathway",

author = "Catherine Rabouille and Kuntz, {Douglas A.} and Anne Lockyer and Rose Watson and Tara Signorelli and Rose, {David R.} and {Van Den Heuvel}, Marcel and Roberts, {David B.}",

year = "1999",

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T1 - The Drosophila GMII gene encodes a Golgi α-mannosidase II

AU - Rabouille, Catherine

AU - Kuntz, Douglas A.

AU - Lockyer, Anne

AU - Watson, Rose

AU - Signorelli, Tara

AU - Rose, David R.

AU - Van Den Heuvel, Marcel

AU - Roberts, David B.

PY - 1999/11/10

Y1 - 1999/11/10

N2 - In this paper we show the organisation of the Drosophila gene encoding a Golgi α-mannosidase II. We demonstrate that it encodes a functional homologue of the mouse Golgi α-mannosidase II. The Drosophila and mouse cDNA sequences translate into amino acid sequences which show 41% identity and 61% similarity. Expression of the Drosophila GMII sequence in CHOP cells produces an enzyme which has mannosidase activity and is inhibited by swainsonine and by CuSO4. In cultured Drosophila cells and in Drosophila embryos, antibodies raised against a C-terminal peptide localise this product mainly to the Golgi apparatus as identified by cryo-immuno electron microscopy studies and by antibodies raised against known mammalian Golgi proteins. We discuss these results in terms of the possible use of dGMII as a Drosophila Golgi marker.

AB - In this paper we show the organisation of the Drosophila gene encoding a Golgi α-mannosidase II. We demonstrate that it encodes a functional homologue of the mouse Golgi α-mannosidase II. The Drosophila and mouse cDNA sequences translate into amino acid sequences which show 41% identity and 61% similarity. Expression of the Drosophila GMII sequence in CHOP cells produces an enzyme which has mannosidase activity and is inhibited by swainsonine and by CuSO4. In cultured Drosophila cells and in Drosophila embryos, antibodies raised against a C-terminal peptide localise this product mainly to the Golgi apparatus as identified by cryo-immuno electron microscopy studies and by antibodies raised against known mammalian Golgi proteins. We discuss these results in terms of the possible use of dGMII as a Drosophila Golgi marker.

KW - α-mannosidase II

KW - Cryo-immuno electron microscopy

KW - Drosophila embryos

KW - Drosophila S2 cell

KW - Genomic sequence

KW - Glycosyl transferase

KW - Golgi apparatus

KW - Indirect immunofluorescence

KW - N-glycosylation pathway

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M3 - Article

C2 - 10504337

AN - SCOPUS:0032704933

SN - 0021-9533

VL - 112

SP - 3319

EP - 3330

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 19

ER -

The Drosophila GMII gene encodes a Golgi α-mannosidase II

Abstract

Keywords

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