The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation

Fulvia Vascotto; Danielle Lankar; Gabrielle Faure-Andre; Pablo Vargas; Jheimmy Diaz; Delphine Le Roux; Maria-Isabel Yuseff; Jean-Baptiste Sibarita; Marianne Boes; Graca Raposo; Evelyne Mougneau; Nicolas Glaichenhaus; Christian Bonnerot; Benedicte Manoury; Ana-Maria Lennon-Dumenil

The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation

Fulvia Vascotto, Danielle Lankar, Gabrielle Faure-Andre, Pablo Vargas, Jheimmy Diaz, Delphine Le Roux, Maria-Isabel Yuseff, Jean-Baptiste Sibarita, Marianne Boes, Graca Raposo, Evelyne Mougneau, Nicolas Glaichenhaus, Christian Bonnerot, Benedicte Manoury, Ana-Maria Lennon-Dumenil^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Antigen (Ag) capture and presentation onto major histocompatibility complex (MHC) class II molecules by B lymphocytes is mediated by their surface Ag receptor (B cell receptor [BCR]). Therefore, the transport of vesicles that carry MHC class II and BCR-Ag complexes must be coordinated for them to converge for processing. In this study, we identify the actin-associated motor protein myosin II as being essential for this process. Myosin II is activated upon BCR engagement and associates with MHC class II-invariant chain complexes. Myosin II inhibition or depletion compromises the convergence and concentration of MHC class II and BCR-Ag complexes into lysosomes devoted to Ag processing. Accordingly, the formation of MHC class II-peptides and subsequent CD4 T cell activation are impaired in cells lacking myosin II activity. Therefore, myosin II emerges as a key motor protein in BCR-driven Ag processing and presentation.

Original language	English
Pages (from-to)	1007-1019
Number of pages	13
Journal	Journal of Cell Biology
Volume	176
Issue number	7
Publication status	Published - Mar 2007

Keywords

B-CELL ACTIVATION
DENDRITIC CELLS
LIPID RAFTS
SYNAPSE FORMATION
PRESENTATION PATHWAY
LATE ENDOSOMES
RECEPTOR
MOLECULES
TRANSPORT
COMPARTMENTS

Cite this

Vascotto, F., Lankar, D., Faure-Andre, G., Vargas, P., Diaz, J., Le Roux, D., Yuseff, M.-I., Sibarita, J.-B., Boes, M., Raposo, G., Mougneau, E., Glaichenhaus, N., Bonnerot, C., Manoury, B., & Lennon-Dumenil, A.-M. (2007). The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation. Journal of Cell Biology, 176(7), 1007-1019.

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title = "The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation",

abstract = "Antigen (Ag) capture and presentation onto major histocompatibility complex (MHC) class II molecules by B lymphocytes is mediated by their surface Ag receptor (B cell receptor [BCR]). Therefore, the transport of vesicles that carry MHC class II and BCR-Ag complexes must be coordinated for them to converge for processing. In this study, we identify the actin-associated motor protein myosin II as being essential for this process. Myosin II is activated upon BCR engagement and associates with MHC class II-invariant chain complexes. Myosin II inhibition or depletion compromises the convergence and concentration of MHC class II and BCR-Ag complexes into lysosomes devoted to Ag processing. Accordingly, the formation of MHC class II-peptides and subsequent CD4 T cell activation are impaired in cells lacking myosin II activity. Therefore, myosin II emerges as a key motor protein in BCR-driven Ag processing and presentation.",

keywords = "B-CELL ACTIVATION, DENDRITIC CELLS, LIPID RAFTS, SYNAPSE FORMATION, PRESENTATION PATHWAY, LATE ENDOSOMES, RECEPTOR, MOLECULES, TRANSPORT, COMPARTMENTS",

author = "Fulvia Vascotto and Danielle Lankar and Gabrielle Faure-Andre and Pablo Vargas and Jheimmy Diaz and {Le Roux}, Delphine and Maria-Isabel Yuseff and Jean-Baptiste Sibarita and Marianne Boes and Graca Raposo and Evelyne Mougneau and Nicolas Glaichenhaus and Christian Bonnerot and Benedicte Manoury and Ana-Maria Lennon-Dumenil",

year = "2007",

month = mar,

language = "English",

volume = "176",

pages = "1007--1019",

journal = "Journal of Cell Biology",

issn = "0021-9525",

publisher = "Rockefeller University Press",

number = "7",

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Vascotto, F, Lankar, D, Faure-Andre, G, Vargas, P, Diaz, J, Le Roux, D, Yuseff, M-I, Sibarita, J-B, Boes, M, Raposo, G, Mougneau, E, Glaichenhaus, N, Bonnerot, C, Manoury, B & Lennon-Dumenil, A-M 2007, 'The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation', Journal of Cell Biology, vol. 176, no. 7, pp. 1007-1019.

TY - JOUR

T1 - The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation

AU - Vascotto, Fulvia

AU - Lankar, Danielle

AU - Faure-Andre, Gabrielle

AU - Vargas, Pablo

AU - Diaz, Jheimmy

AU - Le Roux, Delphine

AU - Yuseff, Maria-Isabel

AU - Sibarita, Jean-Baptiste

AU - Boes, Marianne

AU - Raposo, Graca

AU - Mougneau, Evelyne

AU - Glaichenhaus, Nicolas

AU - Bonnerot, Christian

AU - Manoury, Benedicte

AU - Lennon-Dumenil, Ana-Maria

PY - 2007/3

Y1 - 2007/3

N2 - Antigen (Ag) capture and presentation onto major histocompatibility complex (MHC) class II molecules by B lymphocytes is mediated by their surface Ag receptor (B cell receptor [BCR]). Therefore, the transport of vesicles that carry MHC class II and BCR-Ag complexes must be coordinated for them to converge for processing. In this study, we identify the actin-associated motor protein myosin II as being essential for this process. Myosin II is activated upon BCR engagement and associates with MHC class II-invariant chain complexes. Myosin II inhibition or depletion compromises the convergence and concentration of MHC class II and BCR-Ag complexes into lysosomes devoted to Ag processing. Accordingly, the formation of MHC class II-peptides and subsequent CD4 T cell activation are impaired in cells lacking myosin II activity. Therefore, myosin II emerges as a key motor protein in BCR-driven Ag processing and presentation.

AB - Antigen (Ag) capture and presentation onto major histocompatibility complex (MHC) class II molecules by B lymphocytes is mediated by their surface Ag receptor (B cell receptor [BCR]). Therefore, the transport of vesicles that carry MHC class II and BCR-Ag complexes must be coordinated for them to converge for processing. In this study, we identify the actin-associated motor protein myosin II as being essential for this process. Myosin II is activated upon BCR engagement and associates with MHC class II-invariant chain complexes. Myosin II inhibition or depletion compromises the convergence and concentration of MHC class II and BCR-Ag complexes into lysosomes devoted to Ag processing. Accordingly, the formation of MHC class II-peptides and subsequent CD4 T cell activation are impaired in cells lacking myosin II activity. Therefore, myosin II emerges as a key motor protein in BCR-driven Ag processing and presentation.

KW - B-CELL ACTIVATION

KW - DENDRITIC CELLS

KW - LIPID RAFTS

KW - SYNAPSE FORMATION

KW - PRESENTATION PATHWAY

KW - LATE ENDOSOMES

KW - RECEPTOR

KW - MOLECULES

KW - TRANSPORT

KW - COMPARTMENTS

M3 - Article

SN - 0021-9525

VL - 176

SP - 1007

EP - 1019

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 7

ER -

The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation

Abstract

Keywords

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