Target-selected enu mutagenesis to develop cancer models in the rat

Bart M.G. Smits; Edwin Cuppen; Michael N. Gould

doi:10.1007/978-0-387-69805-2_6

Target-selected enu mutagenesis to develop cancer models in the rat

Bart M.G. Smits
, Edwin Cuppen
, Michael N. Gould^*

^*Corresponding author for this work

Center for Molecular Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

2 Citations (Scopus)

Abstract

Over the last decade, the laboratory rat has matured as a genetic model organism. One of the most valuable additions to its genetic toolbox is the ability to inactivate genes of interest using N-ethyl-N-nitrosourea mutagenesis-based reverse genetics. The Gould lab (McArdle Laboratory for Cancer Research, University of Wisconsin – Madison, USA) and the Cuppen lab (Hubrecht Institute for Developmental Biology and Stem Cell Research, Utrecht, The Netherlands) have developed this technology for a variety of rat strains. This resulted in the identification and characterization of a number of novel rat models, including novel models for breast and colon cancer. This chapter describes the establishment of the rat knockout technology and the characterization of the rat knockout models for the tumor suppressor genes APC, BRCA1, BRCA2, and MSH6.

Original language	English
Title of host publication	Genetically Engineered Mice for Cancer Research
Subtitle of host publication	Design, Analysis, Pathways, Validation and Pre-Clinical Testing
Publisher	Springer New York
Pages	113-131
Number of pages	19
ISBN (Electronic)	9780387698052
ISBN (Print)	9780387698038
DOIs	https://doi.org/10.1007/978-0-387-69805-2_6
Publication status	Published - 1 Jan 2012

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10.1007/978-0-387-69805-2_6

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abstract = "Over the last decade, the laboratory rat has matured as a genetic model organism. One of the most valuable additions to its genetic toolbox is the ability to inactivate genes of interest using N-ethyl-N-nitrosourea mutagenesis-based reverse genetics. The Gould lab (McArdle Laboratory for Cancer Research, University of Wisconsin {\textendash} Madison, USA) and the Cuppen lab (Hubrecht Institute for Developmental Biology and Stem Cell Research, Utrecht, The Netherlands) have developed this technology for a variety of rat strains. This resulted in the identification and characterization of a number of novel rat models, including novel models for breast and colon cancer. This chapter describes the establishment of the rat knockout technology and the characterization of the rat knockout models for the tumor suppressor genes APC, BRCA1, BRCA2, and MSH6.",

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Target-selected enu mutagenesis to develop cancer models in the rat. / Smits, Bart M.G.; Cuppen, Edwin; Gould, Michael N.
Genetically Engineered Mice for Cancer Research: Design, Analysis, Pathways, Validation and Pre-Clinical Testing. Springer New York, 2012. p. 113-131.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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Target-selected enu mutagenesis to develop cancer models in the rat

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