T cell receptor a chain variable (TCR V alpha) genes on mRNA level

H Hu; R de Weger; H J Schuurman

T cell receptor a chain variable (TCR V alpha) genes on mRNA level

Translated title of the contribution: Positive identification of the specificity of polymerase chain reaction product

H Hu, R de Weger, H J Schuurman

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A method of positive identification of the specificity of polymerase chain reaction (PCR) product using internal oligonucleotide probe is introduced. The hybridization was done on the agarose gel which was dried after electrophoresis. Detection of the expression of T cell receptor a chain variable (TCR V alpha) genes on mRNA level was used as the experimental model. Twenty nine TCR V alpha gene subfamilies could be distinguished clearly in healthy human peripheral blood lymphocytes by this method. Positive identification of PCR product on dried agarose gel by internal oligonucleotide probe is relatively simple and less time consuming.

Translated title of the contribution	Positive identification of the specificity of polymerase chain reaction product
Original language	Chinese
Pages (from-to)	435-8
Number of pages	4
Journal	Huaxi Yike Daxue xuebao
Volume	24
Issue number	4
Publication status	Published - Dec 1993

Keywords

Base Sequence
Humans
Molecular Sequence Data
Oligonucleotide Probes
Polymerase Chain Reaction
RNA, Messenger
Receptors, Antigen, T-Cell, alpha-beta
Sensitivity and Specificity

Cite this

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title = "T cell receptor a chain variable (TCR V alpha) genes on mRNA level",

abstract = "A method of positive identification of the specificity of polymerase chain reaction (PCR) product using internal oligonucleotide probe is introduced. The hybridization was done on the agarose gel which was dried after electrophoresis. Detection of the expression of T cell receptor a chain variable (TCR V alpha) genes on mRNA level was used as the experimental model. Twenty nine TCR V alpha gene subfamilies could be distinguished clearly in healthy human peripheral blood lymphocytes by this method. Positive identification of PCR product on dried agarose gel by internal oligonucleotide probe is relatively simple and less time consuming.",

keywords = "Base Sequence, Humans, Molecular Sequence Data, Oligonucleotide Probes, Polymerase Chain Reaction, RNA, Messenger, Receptors, Antigen, T-Cell, alpha-beta, Sensitivity and Specificity",

author = "H Hu and \{de Weger\}, R and Schuurman, \{H J\}",

year = "1993",

month = dec,

language = "Chinese",

volume = "24",

pages = "435--8",

journal = "Huaxi Yike Daxue xuebao",

issn = "0257-7712",

publisher = "West China University of Medical Sciences",

number = "4",

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TY - JOUR

T1 - T cell receptor a chain variable (TCR V alpha) genes on mRNA level

AU - Hu, H

AU - de Weger, R

AU - Schuurman, H J

PY - 1993/12

Y1 - 1993/12

N2 - A method of positive identification of the specificity of polymerase chain reaction (PCR) product using internal oligonucleotide probe is introduced. The hybridization was done on the agarose gel which was dried after electrophoresis. Detection of the expression of T cell receptor a chain variable (TCR V alpha) genes on mRNA level was used as the experimental model. Twenty nine TCR V alpha gene subfamilies could be distinguished clearly in healthy human peripheral blood lymphocytes by this method. Positive identification of PCR product on dried agarose gel by internal oligonucleotide probe is relatively simple and less time consuming.

AB - A method of positive identification of the specificity of polymerase chain reaction (PCR) product using internal oligonucleotide probe is introduced. The hybridization was done on the agarose gel which was dried after electrophoresis. Detection of the expression of T cell receptor a chain variable (TCR V alpha) genes on mRNA level was used as the experimental model. Twenty nine TCR V alpha gene subfamilies could be distinguished clearly in healthy human peripheral blood lymphocytes by this method. Positive identification of PCR product on dried agarose gel by internal oligonucleotide probe is relatively simple and less time consuming.

KW - Base Sequence

KW - Humans

KW - Molecular Sequence Data

KW - Oligonucleotide Probes

KW - Polymerase Chain Reaction

KW - RNA, Messenger

KW - Receptors, Antigen, T-Cell, alpha-beta

KW - Sensitivity and Specificity

M3 - Article

C2 - 8150451

SN - 0257-7712

VL - 24

SP - 435

EP - 438

JO - Huaxi Yike Daxue xuebao

JF - Huaxi Yike Daxue xuebao

IS - 4

ER -

T cell receptor a chain variable (TCR V alpha) genes on mRNA level

Abstract

Keywords

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