TY - JOUR
T1 - Systemic signature of the lung response to respiratory syncytial virus infection
AU - Pennings, Jeroen L A
AU - Schuurhof, Annemieke
AU - Hodemaekers, Hennie M.
AU - Buisman, Annemarie
AU - de Rond, Lia C G H
AU - Widjojoatmodjo, Myra N.
AU - Luytjes, Willem
AU - Kimpen, Jan L L
AU - Bont, Louis
AU - Janssen, Riny
PY - 2011
Y1 - 2011
N2 - Respiratory Syncytial Virus is a frequent cause of severe bronchiolitis in children. To improve our understanding of systemic host responses to RSV, we compared BALB/c mouse gene expression responses at day 1, 2, and 5 during primary RSV infection in lung, bronchial lymph nodes, and blood. We identified a set of 53 interferon-associated and innate immunity genes that give correlated responses in all three murine tissues. Additionally, we identified blood gene signatures that are indicative of acute infection, secondary immune response, and vaccine-enhanced disease, respectively. Eosinophil-associated ribonucleases were characteristic for the vaccine-enhanced disease blood signature. These results indicate that it may be possible to distinguish protective and unfavorable patient lung responses via blood diagnostics.
AB - Respiratory Syncytial Virus is a frequent cause of severe bronchiolitis in children. To improve our understanding of systemic host responses to RSV, we compared BALB/c mouse gene expression responses at day 1, 2, and 5 during primary RSV infection in lung, bronchial lymph nodes, and blood. We identified a set of 53 interferon-associated and innate immunity genes that give correlated responses in all three murine tissues. Additionally, we identified blood gene signatures that are indicative of acute infection, secondary immune response, and vaccine-enhanced disease, respectively. Eosinophil-associated ribonucleases were characteristic for the vaccine-enhanced disease blood signature. These results indicate that it may be possible to distinguish protective and unfavorable patient lung responses via blood diagnostics.
KW - Animals
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Lung/metabolism
KW - Lymph Nodes/metabolism
KW - Mice
KW - Mice, Inbred BALB C
KW - Respiratory Syncytial Virus Infections/blood
KW - Respiratory Syncytial Viruses/physiology
KW - Vaccination
UR - http://www.scopus.com/inward/record.url?scp=79959585798&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0021461
DO - 10.1371/journal.pone.0021461
M3 - Article
C2 - 21731757
AN - SCOPUS:79959585798
SN - 1932-6203
VL - 6
JO - PLoS ONE [E]
JF - PLoS ONE [E]
IS - 6
M1 - e21461
ER -