TY - JOUR
T1 - Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic golgi fragments in vitro
AU - Rabouille, Catherine
AU - Kondo, Hisao
AU - Newman, Richard
AU - Hui, Norman
AU - Freemont, Paul
AU - Warren, Graham
PY - 1998/3/6
Y1 - 1998/3/6
N2 - A cell-free system that mimics the reassembly of Golgi stacks at the end of mitosis requires two ATPases, NSF and p97, to rebuild Golgi cisternae. Morphological studies now show that α-SNAP, a component of the NSF pathway, can inhibit the p97 pathway, whereas p47, a component of the p97 pathway, can inhibit the NSF pathway. Anti-syntaxin 5 antibodies and a soluble, recombinant syntaxin 5 inhibited both pathways, suggesting that this t-SNARE is a common component. Biochemical studies confirmed this, showing that p47 binds directly to syntaxin 5 and competes for binding with α-SNAP. p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to α-SNAP, which mediates the binding of NSF.
AB - A cell-free system that mimics the reassembly of Golgi stacks at the end of mitosis requires two ATPases, NSF and p97, to rebuild Golgi cisternae. Morphological studies now show that α-SNAP, a component of the NSF pathway, can inhibit the p97 pathway, whereas p47, a component of the p97 pathway, can inhibit the NSF pathway. Anti-syntaxin 5 antibodies and a soluble, recombinant syntaxin 5 inhibited both pathways, suggesting that this t-SNARE is a common component. Biochemical studies confirmed this, showing that p47 binds directly to syntaxin 5 and competes for binding with α-SNAP. p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to α-SNAP, which mediates the binding of NSF.
UR - http://www.scopus.com/inward/record.url?scp=0032489499&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81128-9
DO - 10.1016/S0092-8674(00)81128-9
M3 - Article
C2 - 9506515
AN - SCOPUS:0032489499
SN - 0092-8674
VL - 92
SP - 603
EP - 610
JO - Cell
JF - Cell
IS - 5
ER -