Supporting roles of platelet thrombospondin-1 and CD36 in thrombus formation on collagen.

Marijke J E Kuijpers; Susanne De Witt; R. Nergiz-Unal; Roger Van Kruchten; Suzanne Korporaal; Peter Verhamme; Maria Febbraio; Marc Tjwa; Peter J. Voshol; Marc F. Hoylaerts; Judith M E M Cosemans; Johan W M Heemskerk

doi:10.1161/ATVBAHA.113.302917

Supporting roles of platelet thrombospondin-1 and CD36 in thrombus formation on collagen.

Marijke J E Kuijpers, Susanne De Witt, R. Nergiz-Unal, Roger Van Kruchten, Suzanne Korporaal, Peter Verhamme, Maria Febbraio, Marc Tjwa, Peter J. Voshol, Marc F. Hoylaerts, Judith M E M Cosemans, Johan W M Heemskerk

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

OBJECTIVE: Platelets abundantly express the membrane receptor CD36 and store its ligand thrombospondin-1 (TSP1) in the α-granules. We investigated whether released TSP1 can support platelet adhesion and thrombus formation via interaction with CD36.
APPROACH AND RESULTS: Mouse platelets deficient in CD36 showed reduced adhesion to TSP1 and subsequent phosphatidylserine expression. Deficiency in either CD36 or TSP1 resulted in markedly increased dissolution of thrombi formed on collagen, although thrombus buildup was unchanged. In mesenteric vessels in vivo, deficiency in CD36 prolonged the time to occlusion and enhanced embolization, which was in agreement with earlier observations in TSP1-deficient mice. Thrombi formed using wild-type blood stained positively for secreted TSP1. Releasate from wild-type but not from TSP1-deficient platelets enhanced platelet activation, phosphatidylserine expression, and thrombus formation on collagen. The enhancement was dependent on CD36 because it was without effect on thrombus formation by CD36-deficient platelets.
CONCLUSIONS: These results demonstrate an anchoring role of platelet-released TSP1 via CD36 in platelet adhesion and collagen-dependent thrombus stabilization. Thus, the TSP1-CD36 tandem is another platelet ligand-receptor axis contributing to the maintenance of a stable thrombus

Original language	English
Pages (from-to)	1187-1192
Number of pages	6
Journal	Arteriosclerosis, Thrombosis and Vascular Biology
Volume	34
Issue number	6
DOIs	https://doi.org/10.1161/ATVBAHA.113.302917
Publication status	Published - 1 Jun 2014

Keywords

CD36
blood platelets
mice
thrombosis
thrombospondin 1

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10.1161/ATVBAHA.113.302917

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Kuijpers, M. J. E., De Witt, S., Nergiz-Unal, R., Van Kruchten, R., Korporaal, S., Verhamme, P., Febbraio, M., Tjwa, M., Voshol, P. J., Hoylaerts, M. F., Cosemans, J. M. E. M., & Heemskerk, J. W. M. (2014). Supporting roles of platelet thrombospondin-1 and CD36 in thrombus formation on collagen. Arteriosclerosis, Thrombosis and Vascular Biology, 34(6), 1187-1192. https://doi.org/10.1161/ATVBAHA.113.302917

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title = "Supporting roles of platelet thrombospondin-1 and CD36 in thrombus formation on collagen.",

abstract = "OBJECTIVE: Platelets abundantly express the membrane receptor CD36 and store its ligand thrombospondin-1 (TSP1) in the α-granules. We investigated whether released TSP1 can support platelet adhesion and thrombus formation via interaction with CD36.APPROACH AND RESULTS: Mouse platelets deficient in CD36 showed reduced adhesion to TSP1 and subsequent phosphatidylserine expression. Deficiency in either CD36 or TSP1 resulted in markedly increased dissolution of thrombi formed on collagen, although thrombus buildup was unchanged. In mesenteric vessels in vivo, deficiency in CD36 prolonged the time to occlusion and enhanced embolization, which was in agreement with earlier observations in TSP1-deficient mice. Thrombi formed using wild-type blood stained positively for secreted TSP1. Releasate from wild-type but not from TSP1-deficient platelets enhanced platelet activation, phosphatidylserine expression, and thrombus formation on collagen. The enhancement was dependent on CD36 because it was without effect on thrombus formation by CD36-deficient platelets.CONCLUSIONS: These results demonstrate an anchoring role of platelet-released TSP1 via CD36 in platelet adhesion and collagen-dependent thrombus stabilization. Thus, the TSP1-CD36 tandem is another platelet ligand-receptor axis contributing to the maintenance of a stable thrombus",

keywords = "CD36, blood platelets, mice, thrombosis, thrombospondin 1",

author = "Kuijpers, {Marijke J E} and {De Witt}, Susanne and R. Nergiz-Unal and {Van Kruchten}, Roger and Suzanne Korporaal and Peter Verhamme and Maria Febbraio and Marc Tjwa and Voshol, {Peter J.} and Hoylaerts, {Marc F.} and Cosemans, {Judith M E M} and Heemskerk, {Johan W M}",

year = "2014",

month = jun,

day = "1",

doi = "10.1161/ATVBAHA.113.302917",

language = "English",

volume = "34",

pages = "1187--1192",

journal = "Arteriosclerosis, Thrombosis and Vascular Biology",

issn = "1079-5642",

publisher = "Lippincott Williams & Wilkins",

number = "6",

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Kuijpers, MJE, De Witt, S, Nergiz-Unal, R, Van Kruchten, R, Korporaal, S, Verhamme, P, Febbraio, M, Tjwa, M, Voshol, PJ, Hoylaerts, MF, Cosemans, JMEM & Heemskerk, JWM 2014, 'Supporting roles of platelet thrombospondin-1 and CD36 in thrombus formation on collagen.', Arteriosclerosis, Thrombosis and Vascular Biology, vol. 34, no. 6, pp. 1187-1192. https://doi.org/10.1161/ATVBAHA.113.302917

TY - JOUR

T1 - Supporting roles of platelet thrombospondin-1 and CD36 in thrombus formation on collagen.

AU - Kuijpers, Marijke J E

AU - De Witt, Susanne

AU - Nergiz-Unal, R.

AU - Van Kruchten, Roger

AU - Korporaal, Suzanne

AU - Verhamme, Peter

AU - Febbraio, Maria

AU - Tjwa, Marc

AU - Voshol, Peter J.

AU - Hoylaerts, Marc F.

AU - Cosemans, Judith M E M

AU - Heemskerk, Johan W M

PY - 2014/6/1

Y1 - 2014/6/1

N2 - OBJECTIVE: Platelets abundantly express the membrane receptor CD36 and store its ligand thrombospondin-1 (TSP1) in the α-granules. We investigated whether released TSP1 can support platelet adhesion and thrombus formation via interaction with CD36.APPROACH AND RESULTS: Mouse platelets deficient in CD36 showed reduced adhesion to TSP1 and subsequent phosphatidylserine expression. Deficiency in either CD36 or TSP1 resulted in markedly increased dissolution of thrombi formed on collagen, although thrombus buildup was unchanged. In mesenteric vessels in vivo, deficiency in CD36 prolonged the time to occlusion and enhanced embolization, which was in agreement with earlier observations in TSP1-deficient mice. Thrombi formed using wild-type blood stained positively for secreted TSP1. Releasate from wild-type but not from TSP1-deficient platelets enhanced platelet activation, phosphatidylserine expression, and thrombus formation on collagen. The enhancement was dependent on CD36 because it was without effect on thrombus formation by CD36-deficient platelets.CONCLUSIONS: These results demonstrate an anchoring role of platelet-released TSP1 via CD36 in platelet adhesion and collagen-dependent thrombus stabilization. Thus, the TSP1-CD36 tandem is another platelet ligand-receptor axis contributing to the maintenance of a stable thrombus

AB - OBJECTIVE: Platelets abundantly express the membrane receptor CD36 and store its ligand thrombospondin-1 (TSP1) in the α-granules. We investigated whether released TSP1 can support platelet adhesion and thrombus formation via interaction with CD36.APPROACH AND RESULTS: Mouse platelets deficient in CD36 showed reduced adhesion to TSP1 and subsequent phosphatidylserine expression. Deficiency in either CD36 or TSP1 resulted in markedly increased dissolution of thrombi formed on collagen, although thrombus buildup was unchanged. In mesenteric vessels in vivo, deficiency in CD36 prolonged the time to occlusion and enhanced embolization, which was in agreement with earlier observations in TSP1-deficient mice. Thrombi formed using wild-type blood stained positively for secreted TSP1. Releasate from wild-type but not from TSP1-deficient platelets enhanced platelet activation, phosphatidylserine expression, and thrombus formation on collagen. The enhancement was dependent on CD36 because it was without effect on thrombus formation by CD36-deficient platelets.CONCLUSIONS: These results demonstrate an anchoring role of platelet-released TSP1 via CD36 in platelet adhesion and collagen-dependent thrombus stabilization. Thus, the TSP1-CD36 tandem is another platelet ligand-receptor axis contributing to the maintenance of a stable thrombus

KW - CD36

KW - blood platelets

KW - mice

KW - thrombosis

KW - thrombospondin 1

U2 - 10.1161/ATVBAHA.113.302917

DO - 10.1161/ATVBAHA.113.302917

M3 - Article

C2 - 24675658

SN - 1079-5642

VL - 34

SP - 1187

EP - 1192

JO - Arteriosclerosis, Thrombosis and Vascular Biology

JF - Arteriosclerosis, Thrombosis and Vascular Biology

IS - 6

ER -

Supporting roles of platelet thrombospondin-1 and CD36 in thrombus formation on collagen.

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Keywords

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