Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model

I. Filz von Reiterdank, P. Tawa, Y. Berkane, E. de Clermont-Tonnerre, A. T. Dinicu, C. Pendexter, M. Goutard, A. G. Lellouch, A. B. Mink van der Molen, J. H. Coert, C. L. Cetrulo, K. Uygun*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Ischemia is a major limiting factor in Vascularized Composite Allotransplantation (VCA) as irreversible muscular injury can occur after as early as 4–6 h of static cold storage (SCS). Organ preservation technologies have led to the development of storage protocols extending rat liver ex vivo preservation up to 4 days. Development of such a protocol for VCAs has the added challenge of inherent ice nucleating factors of the graft, therefore, this study focused on developing a robust protocol for VCA supercooling. Rodent partial hindlimbs underwent subnormothermic machine perfusion (SNMP) with several loading solutions, followed by a storage solution with cryoprotective agents (CPA) developed for VCAs. Storage occurred in suspended animation for 24h and VCAs were recovered using SNMP with modified Steen. This study shows a robust VCA supercooling preservation protocol in a rodent model. Further optimization is expected to allow for its application in a transplantation model, which would be a breakthrough in the field of VCA preservation.

Original languageEnglish
Article number104950
Number of pages9
JournalCryobiology
Volume116
DOIs
Publication statusPublished - Sept 2024

Keywords

  • Preservation
  • Subzero non-freezing
  • Supercooling
  • Vascularized composite allografts

Fingerprint

Dive into the research topics of 'Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model'. Together they form a unique fingerprint.

Cite this