Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)

A Kaptein; M Jansen; G Dilaver; J Kitson; L Dash; E Wang; M J Owen; J L Bodmer; J Tschopp; S N Farrow

doi:10.1016/s0014-5793(00)02219-5

Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)

A Kaptein, M Jansen, G Dilaver, J Kitson, L Dash, E Wang, M J Owen, J L Bodmer, J Tschopp, S N Farrow

Bachelor Degree Course Biomedical Sciences

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

WSL-1/TRAMP (DR3) is a member of the tumour necrosis factor (TNF) receptor superfamily which exhibits effects on NF-kappaB activation and apoptosis. TWEAK, a novel TNF-related molecule, has been proposed as the ligand for this receptor. Utilising both human and murine TWEAK ligand, it is shown that TWEAK and WSL-1/TRAMP do not interact in an in vitro binding assay and that TWEAK binds strongly to cells that do not express WSL-1/TRAMP on the cell surface. Biological activity of TWEAK is also observed in these cells. Finally, cells isolated from WSL-1/TRAMP knockout mice are shown to retain their ability to interact with TWEAK. These results suggest that WSL-1/TRAMP is not the major receptor for TWEAK

Original language	English
Pages (from-to)	135-41
Number of pages	7
Journal	FEBS letters
Volume	485
Issue number	2-3
DOIs	https://doi.org/10.1016/s0014-5793(00)02219-5
Publication status	Published - 24 Nov 2000

Keywords

Animals
Apoptosis
Apoptosis Regulatory Proteins
Carrier Proteins/metabolism
Cell Line
Cytokine TWEAK
Humans
Interleukin-8/pharmacology
Lymphocytes/metabolism
Mice
Mice, Knockout
NF-kappa B/metabolism
Receptors, Tumor Necrosis Factor/deficiency
Receptors, Tumor Necrosis Factor, Member 25
Tumor Necrosis Factor-alpha/metabolism
Tumor Necrosis Factors

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10.1016/s0014-5793(00)02219-5

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title = "Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)",

abstract = "WSL-1/TRAMP (DR3) is a member of the tumour necrosis factor (TNF) receptor superfamily which exhibits effects on NF-kappaB activation and apoptosis. TWEAK, a novel TNF-related molecule, has been proposed as the ligand for this receptor. Utilising both human and murine TWEAK ligand, it is shown that TWEAK and WSL-1/TRAMP do not interact in an in vitro binding assay and that TWEAK binds strongly to cells that do not express WSL-1/TRAMP on the cell surface. Biological activity of TWEAK is also observed in these cells. Finally, cells isolated from WSL-1/TRAMP knockout mice are shown to retain their ability to interact with TWEAK. These results suggest that WSL-1/TRAMP is not the major receptor for TWEAK",

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author = "A Kaptein and M Jansen and G Dilaver and J Kitson and L Dash and E Wang and Owen, {M J} and Bodmer, {J L} and J Tschopp and Farrow, {S N}",

year = "2000",

month = nov,

day = "24",

doi = "10.1016/s0014-5793(00)02219-5",

language = "English",

volume = "485",

pages = "135--41",

journal = "FEBS letters",

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T1 - Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)

AU - Kaptein, A

AU - Jansen, M

AU - Dilaver, G

AU - Kitson, J

AU - Dash, L

AU - Wang, E

AU - Owen, M J

AU - Bodmer, J L

AU - Tschopp, J

AU - Farrow, S N

PY - 2000/11/24

Y1 - 2000/11/24

N2 - WSL-1/TRAMP (DR3) is a member of the tumour necrosis factor (TNF) receptor superfamily which exhibits effects on NF-kappaB activation and apoptosis. TWEAK, a novel TNF-related molecule, has been proposed as the ligand for this receptor. Utilising both human and murine TWEAK ligand, it is shown that TWEAK and WSL-1/TRAMP do not interact in an in vitro binding assay and that TWEAK binds strongly to cells that do not express WSL-1/TRAMP on the cell surface. Biological activity of TWEAK is also observed in these cells. Finally, cells isolated from WSL-1/TRAMP knockout mice are shown to retain their ability to interact with TWEAK. These results suggest that WSL-1/TRAMP is not the major receptor for TWEAK

AB - WSL-1/TRAMP (DR3) is a member of the tumour necrosis factor (TNF) receptor superfamily which exhibits effects on NF-kappaB activation and apoptosis. TWEAK, a novel TNF-related molecule, has been proposed as the ligand for this receptor. Utilising both human and murine TWEAK ligand, it is shown that TWEAK and WSL-1/TRAMP do not interact in an in vitro binding assay and that TWEAK binds strongly to cells that do not express WSL-1/TRAMP on the cell surface. Biological activity of TWEAK is also observed in these cells. Finally, cells isolated from WSL-1/TRAMP knockout mice are shown to retain their ability to interact with TWEAK. These results suggest that WSL-1/TRAMP is not the major receptor for TWEAK

KW - Animals

KW - Apoptosis

KW - Apoptosis Regulatory Proteins

KW - Carrier Proteins/metabolism

KW - Cell Line

KW - Cytokine TWEAK

KW - Humans

KW - Interleukin-8/pharmacology

KW - Lymphocytes/metabolism

KW - Mice

KW - Mice, Knockout

KW - NF-kappa B/metabolism

KW - Receptors, Tumor Necrosis Factor/deficiency

KW - Receptors, Tumor Necrosis Factor, Member 25

KW - Tumor Necrosis Factor-alpha/metabolism

KW - Tumor Necrosis Factors

U2 - 10.1016/s0014-5793(00)02219-5

DO - 10.1016/s0014-5793(00)02219-5

M3 - Article

C2 - 11094155

SN - 0014-5793

VL - 485

SP - 135

EP - 141

JO - FEBS letters

JF - FEBS letters

IS - 2-3

ER -

Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)

Abstract

Keywords

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