TY - JOUR
T1 - Stable and Antibacterial Magnesium-Graphene Nanocomposite-Based Implants for Bone Repair
AU - Safari, Narges
AU - Golafshan, Nasim
AU - Kharaziha, Mahshid
AU - Toroghinejad, Mohammad Reza
AU - Utomo, Lizette
AU - Malda, Jos
AU - Castilho, Miguel
N1 - Funding Information:
The research leading to these results has received financial support from Isfahan University of Technology. M.C. and J.M. acknowledge the partners of Regenerative Medicine Crossing Borders ( www.regmedxb.com ) powered by Health ∼ Holland, Top Sector Life Sciences & Health, as well as the support of the Dutch Arthritis Foundation (LLP-12 and LLP-22). In addition, the authors are very grateful to Inge Dokter for all of the support with the cell harvesting, in vitro culture, and gene expression analysis.
Publisher Copyright:
© 2020 American Chemical Society. All rights reserved.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020/11/9
Y1 - 2020/11/9
N2 - Magnesium (Mg)-based alloys are promising biodegradable materials for bone repair applications. However, due to their rapid degradation and high corrosion rate, Mg-based alloys are typically associated with in vivo infections and implant failure. This study evaluated the synergistic stability and anti-inflammatory properties that could potentially be achieved by the modification of the Mg alloy with graphene nanoparticles (Gr). Incorporation of low dosages of Gr (0.18 and 0.50 wt %) in a Mg alloy with aluminum (Al, 1 wt %) and copper (Cu, 0.25 wt %) was successfully achieved by a spark plasma sintering (SPS) method. Notably, the degradation rate of the Mg-based alloys was reduced approximately 4-fold and the bactericidal activity was enhanced up to 5-fold with incorporation of only 0.18 wt % Gr to the Mg-1Al-Cu matrix. Moreover, the modified Mg-based nanocomposites with 0.18 wt % Gr demonstrated compressive properties within the range of native cancellous bone (modulus of approximately 6 GPa), whereas in vitro studies with human mesenchymal stromal cells (hMSCs) showed high cytocompatibility and superior osteogenic properties compared to non-Gr-modified Mg-1Al-Cu implants. Overall, this study provides foundations for the fabrication of stable, yet fully resorbable, Mg-based bone implants that could reduce implant-associated infections.
AB - Magnesium (Mg)-based alloys are promising biodegradable materials for bone repair applications. However, due to their rapid degradation and high corrosion rate, Mg-based alloys are typically associated with in vivo infections and implant failure. This study evaluated the synergistic stability and anti-inflammatory properties that could potentially be achieved by the modification of the Mg alloy with graphene nanoparticles (Gr). Incorporation of low dosages of Gr (0.18 and 0.50 wt %) in a Mg alloy with aluminum (Al, 1 wt %) and copper (Cu, 0.25 wt %) was successfully achieved by a spark plasma sintering (SPS) method. Notably, the degradation rate of the Mg-based alloys was reduced approximately 4-fold and the bactericidal activity was enhanced up to 5-fold with incorporation of only 0.18 wt % Gr to the Mg-1Al-Cu matrix. Moreover, the modified Mg-based nanocomposites with 0.18 wt % Gr demonstrated compressive properties within the range of native cancellous bone (modulus of approximately 6 GPa), whereas in vitro studies with human mesenchymal stromal cells (hMSCs) showed high cytocompatibility and superior osteogenic properties compared to non-Gr-modified Mg-1Al-Cu implants. Overall, this study provides foundations for the fabrication of stable, yet fully resorbable, Mg-based bone implants that could reduce implant-associated infections.
KW - antibacterial properties
KW - bone implants
KW - corrosion resistance
KW - degradation
KW - magnesium alloys
UR - http://www.scopus.com/inward/record.url?scp=85097500440&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.0c00613
DO - 10.1021/acsbiomaterials.0c00613
M3 - Article
SN - 2373-9878
VL - 6
SP - 6253
EP - 6262
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 11
ER -