TY - JOUR
T1 - Spread of old and new clones of epidemic methicillin-resistant Staphylococcus aureus in Poland
AU - Murchan, Stephen
AU - Trzciñski, Krzysztof
AU - Skoczyñska, Anna
AU - Van Leeuwen, Willem
AU - Van Belkum, Alex
AU - Pietuszko, Slawomir
AU - Gadomski, Tadeusz
AU - Hryniewicz, Waleria
PY - 1998/1/1
Y1 - 1998/1/1
N2 - Objective: To study the relatedness among methicillin-resistant Staphylococcus aureus (MRSA) isolates originating from two regions of Poland using different epidemiologic typing methods. Methods: Forty-five MRSA isolates (19 from Warsaw and 26 from the Grajewo region) were collected between 1995 and 1996. For phenotypic epidemiologic analysis, antimicrobial susceptibility testing (AST) with a panel of 19 antibiotics was performed. For genotypic epidemiologic analysis, pulsed-field gel electrophoresis (PFGE) of SmaI-digested chromosomal DNA, restriction endonuclease analysis of plasmid (REAP) DNA digested by HindIII, random amplification of polymorphic DNA (RAPD) and binary typing (BT) of genomic DNA by hybridization with five different RAPD-generated strain-specific DNA probes, were used. Results: Six clusters of clonally related strains were found among the MRSA isolates analyzed. Three of these, identified in both regions, were related to previously described Polish epidemic clones, designated HeEMRSA-Pol1 (heterogeneously methicillin resistant - 18 isolates) and HoEMRSA-Pol1 (homogeneously resistant - two clones, six isolates each). The remaining three clones, identified in the Grajewo region only, are previously undescribed. One of these, represented by 11 isolates, appears to be new epidemic heterogeneous MRSA clone (HeEMRSA-Pol2). Results of PFGE and BT in general showed good correlation, and, in some cases, RAPD using AP1 and AP7 primers could discriminate between isolates belonging to single PFGE or BT types. Broad AST and REAP can provide useful additional information concerning relatedness. Conclusion: Evidence for the spread of previously recognized epidemic MRSA clones in Poland and the presence of a new epidemic heterogeneously resistant clone of MRSA in hospitals outside Warsaw is documented.
AB - Objective: To study the relatedness among methicillin-resistant Staphylococcus aureus (MRSA) isolates originating from two regions of Poland using different epidemiologic typing methods. Methods: Forty-five MRSA isolates (19 from Warsaw and 26 from the Grajewo region) were collected between 1995 and 1996. For phenotypic epidemiologic analysis, antimicrobial susceptibility testing (AST) with a panel of 19 antibiotics was performed. For genotypic epidemiologic analysis, pulsed-field gel electrophoresis (PFGE) of SmaI-digested chromosomal DNA, restriction endonuclease analysis of plasmid (REAP) DNA digested by HindIII, random amplification of polymorphic DNA (RAPD) and binary typing (BT) of genomic DNA by hybridization with five different RAPD-generated strain-specific DNA probes, were used. Results: Six clusters of clonally related strains were found among the MRSA isolates analyzed. Three of these, identified in both regions, were related to previously described Polish epidemic clones, designated HeEMRSA-Pol1 (heterogeneously methicillin resistant - 18 isolates) and HoEMRSA-Pol1 (homogeneously resistant - two clones, six isolates each). The remaining three clones, identified in the Grajewo region only, are previously undescribed. One of these, represented by 11 isolates, appears to be new epidemic heterogeneous MRSA clone (HeEMRSA-Pol2). Results of PFGE and BT in general showed good correlation, and, in some cases, RAPD using AP1 and AP7 primers could discriminate between isolates belonging to single PFGE or BT types. Broad AST and REAP can provide useful additional information concerning relatedness. Conclusion: Evidence for the spread of previously recognized epidemic MRSA clones in Poland and the presence of a new epidemic heterogeneously resistant clone of MRSA in hospitals outside Warsaw is documented.
KW - Genotyping
KW - Methicillin-resistant staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=0031717955&partnerID=8YFLogxK
U2 - 10.1111/j.1469-0691.1998.tb00402.x
DO - 10.1111/j.1469-0691.1998.tb00402.x
M3 - Article
AN - SCOPUS:0031717955
SN - 1198-743X
VL - 4
SP - 481
EP - 490
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
IS - 9
ER -