Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine

Daisuke Chinda; Toshihiko Takada; Tatsuya Mikami; Kensuke Shimizu; Kosuke Oana; Tetsu Arai; Kazuki Akitaya; Hirotake Sakuraba; Miyuki Katto; Yusuke Nagara; Hiroshi Makino; Daichi Fujii; Kenji Oishi; Shinsaku Fukuda

doi:10.1038/s41598-022-07594-6

Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine

Daisuke Chinda, Toshihiko Takada, Tatsuya Mikami, Kensuke Shimizu, Kosuke Oana, Tetsu Arai, Kazuki Akitaya, Hirotake Sakuraba, Miyuki Katto, Yusuke Nagara, Hiroshi Makino, Daichi Fujii, Kenji Oishi, Shinsaku Fukuda

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Gut microbiomics is based on analysis of both live and dead cells in the stool. However, to understand the ecology of gut microbiota and their symbiotic relationships with hosts, spatial distribution of live bacteria must be examined. Here, we analyzed the live composition of luminal microbiota (LM) and mucosa-associated microbiota (MAM) in the ascending and descending colons and the rectums of 10 healthy adults and compared it with the total composition. The abundance of Lachnospiraceae in live LM decreased along the gut length and was significantly lower than that in total LM. Contrastingly, the abundance of Bacteroidaceae and Bifidobacteriaceae in live LM was higher than that in total LM, suggesting differences in death rate during gut migration. Live Enterobacteriaceae levels in MAM were significantly higher in rectum than in the ascending and descending colons and in LM. High-performance liquid chromatographic analysis of luminal bile acids revealed that 7α-dehydroxylation occurred towards the rectum. In live LM where a bile acid-inducible gene could be detected, 7α-dehydroxylation rates were higher than those in the group without the gene. Overall, we showed differences in live bacteria composition among three gut sites and between LM and MAM, highlighting the importance of understanding their spatial distribution.

Original language	English
Article number	3593
Pages (from-to)	3593
Journal	Scientific Reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-07594-6
Publication status	Published - 4 Mar 2022
Externally published	Yes

Keywords

Adult
Bacteria/genetics
Bile Acids and Salts
Gastrointestinal Microbiome
Humans
Intestine, Large
Microbiota

Access to Document

10.1038/s41598-022-07594-6

Cite this

Chinda, D., Takada, T., Mikami, T., Shimizu, K., Oana, K., Arai, T., Akitaya, K., Sakuraba, H., Katto, M., Nagara, Y., Makino, H., Fujii, D., Oishi, K., & Fukuda, S. (2022). Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine. Scientific Reports, 12(1), 3593. Article 3593. https://doi.org/10.1038/s41598-022-07594-6

@article{fcccb749f5334831b3c089fceed401bb,

title = "Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine",

abstract = "Gut microbiomics is based on analysis of both live and dead cells in the stool. However, to understand the ecology of gut microbiota and their symbiotic relationships with hosts, spatial distribution of live bacteria must be examined. Here, we analyzed the live composition of luminal microbiota (LM) and mucosa-associated microbiota (MAM) in the ascending and descending colons and the rectums of 10 healthy adults and compared it with the total composition. The abundance of Lachnospiraceae in live LM decreased along the gut length and was significantly lower than that in total LM. Contrastingly, the abundance of Bacteroidaceae and Bifidobacteriaceae in live LM was higher than that in total LM, suggesting differences in death rate during gut migration. Live Enterobacteriaceae levels in MAM were significantly higher in rectum than in the ascending and descending colons and in LM. High-performance liquid chromatographic analysis of luminal bile acids revealed that 7α-dehydroxylation occurred towards the rectum. In live LM where a bile acid-inducible gene could be detected, 7α-dehydroxylation rates were higher than those in the group without the gene. Overall, we showed differences in live bacteria composition among three gut sites and between LM and MAM, highlighting the importance of understanding their spatial distribution.",

keywords = "Adult, Bacteria/genetics, Bile Acids and Salts, Gastrointestinal Microbiome, Humans, Intestine, Large, Microbiota",

author = "Daisuke Chinda and Toshihiko Takada and Tatsuya Mikami and Kensuke Shimizu and Kosuke Oana and Tetsu Arai and Kazuki Akitaya and Hirotake Sakuraba and Miyuki Katto and Yusuke Nagara and Hiroshi Makino and Daichi Fujii and Kenji Oishi and Shinsaku Fukuda",

note = "Funding Information: We would like to thank Editage (www.editage.com) for English language editing. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = mar,

day = "4",

doi = "10.1038/s41598-022-07594-6",

language = "English",

volume = "12",

pages = "3593",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine

AU - Chinda, Daisuke

AU - Takada, Toshihiko

AU - Mikami, Tatsuya

AU - Shimizu, Kensuke

AU - Oana, Kosuke

AU - Arai, Tetsu

AU - Akitaya, Kazuki

AU - Sakuraba, Hirotake

AU - Katto, Miyuki

AU - Nagara, Yusuke

AU - Makino, Hiroshi

AU - Fujii, Daichi

AU - Oishi, Kenji

AU - Fukuda, Shinsaku

PY - 2022/3/4

Y1 - 2022/3/4

N2 - Gut microbiomics is based on analysis of both live and dead cells in the stool. However, to understand the ecology of gut microbiota and their symbiotic relationships with hosts, spatial distribution of live bacteria must be examined. Here, we analyzed the live composition of luminal microbiota (LM) and mucosa-associated microbiota (MAM) in the ascending and descending colons and the rectums of 10 healthy adults and compared it with the total composition. The abundance of Lachnospiraceae in live LM decreased along the gut length and was significantly lower than that in total LM. Contrastingly, the abundance of Bacteroidaceae and Bifidobacteriaceae in live LM was higher than that in total LM, suggesting differences in death rate during gut migration. Live Enterobacteriaceae levels in MAM were significantly higher in rectum than in the ascending and descending colons and in LM. High-performance liquid chromatographic analysis of luminal bile acids revealed that 7α-dehydroxylation occurred towards the rectum. In live LM where a bile acid-inducible gene could be detected, 7α-dehydroxylation rates were higher than those in the group without the gene. Overall, we showed differences in live bacteria composition among three gut sites and between LM and MAM, highlighting the importance of understanding their spatial distribution.

AB - Gut microbiomics is based on analysis of both live and dead cells in the stool. However, to understand the ecology of gut microbiota and their symbiotic relationships with hosts, spatial distribution of live bacteria must be examined. Here, we analyzed the live composition of luminal microbiota (LM) and mucosa-associated microbiota (MAM) in the ascending and descending colons and the rectums of 10 healthy adults and compared it with the total composition. The abundance of Lachnospiraceae in live LM decreased along the gut length and was significantly lower than that in total LM. Contrastingly, the abundance of Bacteroidaceae and Bifidobacteriaceae in live LM was higher than that in total LM, suggesting differences in death rate during gut migration. Live Enterobacteriaceae levels in MAM were significantly higher in rectum than in the ascending and descending colons and in LM. High-performance liquid chromatographic analysis of luminal bile acids revealed that 7α-dehydroxylation occurred towards the rectum. In live LM where a bile acid-inducible gene could be detected, 7α-dehydroxylation rates were higher than those in the group without the gene. Overall, we showed differences in live bacteria composition among three gut sites and between LM and MAM, highlighting the importance of understanding their spatial distribution.

KW - Adult

KW - Bacteria/genetics

KW - Bile Acids and Salts

KW - Gastrointestinal Microbiome

KW - Humans

KW - Intestine, Large

KW - Microbiota

UR - http://www.scopus.com/inward/record.url?scp=85125785763&partnerID=8YFLogxK

U2 - 10.1038/s41598-022-07594-6

DO - 10.1038/s41598-022-07594-6

M3 - Article

C2 - 35246580

SN - 2045-2322

VL - 12

SP - 3593

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 3593

ER -

Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this