Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates

Suhail Ahmad; Ziauddin Khan; Ferry Hagen; Jacques F Meis

doi:10.1128/JCM.00408-14

Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates

Suhail Ahmad
, Ziauddin Khan^*
, Ferry Hagen
, Jacques F Meis

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.

Original language	English
Pages (from-to)	2223-7
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00408-14
Publication status	Published - Jun 2014
Externally published	Yes

Keywords

Antifungal Agents/pharmacology
Aspergillus fumigatus/drug effects
Costs and Cost Analysis
Cytochrome P-450 Enzyme System/genetics
Fungal Proteins/genetics
Genotyping Techniques/economics
Humans
Microbial Sensitivity Tests/methods
Mutation, Missense
Polymerase Chain Reaction/economics
Polymorphism, Restriction Fragment Length
Triazoles/pharmacology

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10.1128/JCM.00408-14

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title = "Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates",

abstract = "Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.",

keywords = "Antifungal Agents/pharmacology, Aspergillus fumigatus/drug effects, Costs and Cost Analysis, Cytochrome P-450 Enzyme System/genetics, Fungal Proteins/genetics, Genotyping Techniques/economics, Humans, Microbial Sensitivity Tests/methods, Mutation, Missense, Polymerase Chain Reaction/economics, Polymorphism, Restriction Fragment Length, Triazoles/pharmacology",

author = "Suhail Ahmad and Ziauddin Khan and Ferry Hagen and Meis, \{Jacques F\}",

year = "2014",

month = jun,

doi = "10.1128/JCM.00408-14",

language = "English",

volume = "52",

pages = "2223--7",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "6",

}

TY - JOUR

T1 - Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates

AU - Ahmad, Suhail

AU - Khan, Ziauddin

AU - Hagen, Ferry

AU - Meis, Jacques F

PY - 2014/6

Y1 - 2014/6

N2 - Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.

AB - Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.

KW - Antifungal Agents/pharmacology

KW - Aspergillus fumigatus/drug effects

KW - Costs and Cost Analysis

KW - Cytochrome P-450 Enzyme System/genetics

KW - Fungal Proteins/genetics

KW - Genotyping Techniques/economics

KW - Humans

KW - Microbial Sensitivity Tests/methods

KW - Mutation, Missense

KW - Polymerase Chain Reaction/economics

KW - Polymorphism, Restriction Fragment Length

KW - Triazoles/pharmacology

U2 - 10.1128/JCM.00408-14

DO - 10.1128/JCM.00408-14

M3 - Article

C2 - 24719446

SN - 0095-1137

VL - 52

SP - 2223

EP - 2227

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

ER -

Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates

Abstract

Keywords

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