TY - JOUR
T1 - Signal Inhibitory Receptor on Leukocytes-1 is highly expressed on lung monocytes, but absent on mononuclear phagocytes in skin and colon
AU - von Richthofen, Helen J.
AU - Gollnast, Doron
AU - van Capel, Toni M.M.
AU - Giovannone, Barbara
AU - Westerlaken, Geertje H.A.
AU - Lutter, Lisanne
AU - Oldenburg, Bas
AU - Hijnen, Dirk Jan
AU - van der Vlist, Michiel
AU - de Jong, Esther C.
AU - Meyaard, Linde
N1 - Funding Information:
This work was supported by a Vici grant from the Netherlands Organization for Scientific Research (NWO, grant no. 91815608)
Funding Information:
This work was supported by a Vici grant from the Netherlands Organization for Scientific Research ( NWO , grant no. 91815608 )
Publisher Copyright:
© 2020 The Authors
PY - 2020/11
Y1 - 2020/11
N2 - Signal Inhibitory Receptor on Leukocytes-1 (SIRL-1) is expressed on human blood monocytes and granulocytes and inhibits myeloid effector functions. On monocytes, but not granulocytes, SIRL-1 expression is low or absent in individuals with the single nucleotide polymorphism (SNP) rs612529C. The expression of SIRL-1 in tissue and the influence of rs612529 hereon is currently unknown. Here, we used flow cytometry to determine SIRL-1 expression on immune cells in human blood and three barrier tissues; skin, colon and lung. SIRL-1 was expressed by virtually all neutrophils and eosinophils in these tissues. In contrast, SIRL-1 was not expressed by monocyte-derived cells in skin and colon, whereas it was highly expressed by lung classical monocytes. Lung monocytes from individuals with a rs612529C allele had decreased SIRL-1 expression, consistent with the genotype association in blood. Within the different monocyte subsets in blood and lung, SIRL-1 expression was highest in classical monocytes and lowest in nonclassical monocytes. SIRL-1 was not expressed by dendritic cells in blood and barrier tissues. Together, these results indicate that SIRL-1 is differentially expressed on phagocyte subsets in blood and barrier tissues, and that its expression on monocytes is genotype- and tissue-specific. Immune regulation of monocytes by SIRL-1 may be of particular importance in the lung.
AB - Signal Inhibitory Receptor on Leukocytes-1 (SIRL-1) is expressed on human blood monocytes and granulocytes and inhibits myeloid effector functions. On monocytes, but not granulocytes, SIRL-1 expression is low or absent in individuals with the single nucleotide polymorphism (SNP) rs612529C. The expression of SIRL-1 in tissue and the influence of rs612529 hereon is currently unknown. Here, we used flow cytometry to determine SIRL-1 expression on immune cells in human blood and three barrier tissues; skin, colon and lung. SIRL-1 was expressed by virtually all neutrophils and eosinophils in these tissues. In contrast, SIRL-1 was not expressed by monocyte-derived cells in skin and colon, whereas it was highly expressed by lung classical monocytes. Lung monocytes from individuals with a rs612529C allele had decreased SIRL-1 expression, consistent with the genotype association in blood. Within the different monocyte subsets in blood and lung, SIRL-1 expression was highest in classical monocytes and lowest in nonclassical monocytes. SIRL-1 was not expressed by dendritic cells in blood and barrier tissues. Together, these results indicate that SIRL-1 is differentially expressed on phagocyte subsets in blood and barrier tissues, and that its expression on monocytes is genotype- and tissue-specific. Immune regulation of monocytes by SIRL-1 may be of particular importance in the lung.
KW - Barrier tissue
KW - Granulocyte
KW - Immune checkpoint
KW - Inhibitory receptor
KW - Mononuclear phagocyte
KW - Phagocyte
UR - https://www.scopus.com/pages/publications/85090837961
U2 - 10.1016/j.cellimm.2020.104199
DO - 10.1016/j.cellimm.2020.104199
M3 - Article
C2 - 32942189
AN - SCOPUS:85090837961
SN - 0008-8749
VL - 357
JO - Cellular Immunology
JF - Cellular Immunology
M1 - 104199
ER -