Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

Kleber Y Fertrin; Leigh Samsel; Eduard J van Beers; Laurel Mendelsohn; Gregory J Kato; J Philip McCoy

doi:10.1007/978-1-4939-3302-0_20

Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

Kleber Y Fertrin, Leigh Samsel, Eduard J van Beers, Laurel Mendelsohn, Gregory J Kato, J Philip McCoy

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.

Original language	English
Pages (from-to)	279-292
Number of pages	14
Journal	Methods in molecular biology
Volume	1389
DOIs	https://doi.org/10.1007/978-1-4939-3302-0_20
Publication status	Published - 2016
Externally published	Yes

Keywords

Sickle cell disease
Sickling assay
Imaging flow cytometry

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10.1007/978-1-4939-3302-0_20

Cite this

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title = "Sickle Cell Imaging Flow Cytometry Assay (SIFCA)",

abstract = "Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.",

keywords = "Sickle cell disease , Sickling assay, Imaging flow cytometry",

author = "Fertrin, {Kleber Y} and Leigh Samsel and {van Beers}, {Eduard J} and Laurel Mendelsohn and Kato, {Gregory J} and McCoy, {J Philip}",

note = "Imaging Flow Cytometry : Methods and Protocols. Part V",

year = "2016",

doi = "10.1007/978-1-4939-3302-0_20",

language = "English",

volume = "1389",

pages = "279--292",

journal = "Methods in molecular biology",

issn = "1064-3745",

publisher = "Humana Press",

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T1 - Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

AU - Fertrin, Kleber Y

AU - Samsel, Leigh

AU - van Beers, Eduard J

AU - Mendelsohn, Laurel

AU - Kato, Gregory J

AU - McCoy, J Philip

N1 - Imaging Flow Cytometry : Methods and Protocols. Part V

PY - 2016

Y1 - 2016

N2 - Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.

AB - Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.

KW - Sickle cell disease

KW - Sickling assay

KW - Imaging flow cytometry

U2 - 10.1007/978-1-4939-3302-0_20

DO - 10.1007/978-1-4939-3302-0_20

M3 - Article

C2 - 27460253

SN - 1064-3745

VL - 1389

SP - 279

EP - 292

JO - Methods in molecular biology

JF - Methods in molecular biology

ER -

Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

Abstract

Keywords

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