Serum proteomics reveals hemophagocytic lymphohistiocytosis-like phenotype in a subset of patients with multisystem inflammatory syndrome in children

Adam J Tulling, Marloes G Holierhoek, Anja M Jansen-Hoogendijk, Levi Hoste, Filomeen Haerynck, Simon J Tavernier, Rianne Oostenbrink, Corinne M P Buysse, Michiel A G E Bannier, Jolita Bekhof, Mijke Breukels, Sanne C Hammer, Monique A M Jacobs, Arvid W A Kamps, Jan W van der Linden, Ankie Lebon, Johanna H Oudshoorn, Gerdien A Tramper-Stranders, Sebastiaan J Vastert, Jantien W WieringaSuzanne W J Terheggen-Lagro, Joanne G Wildenbeest, Erik G J von Asmuth, Erik B van den Akker, Marielle E van Gijn, Gertjan Lugthart, Emilie P Buddingh*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Children with Multisystem Inflammatory Syndrome in Children (MIS-C) can present with thrombocytopenia, which is a key feature of hemophagocytic lymphohistiocytosis (HLH). We hypothesized that thrombocytopenic MIS-C patients have more features of HLH. Clinical characteristics and routine laboratory parameters were collected from 228 MIS-C patients, of whom 85 (37%) were thrombocytopenic. Thrombocytopenic patients had increased ferritin levels; reduced leukocyte subsets; and elevated levels of ASAT and ALAT. Soluble IL-2RA was higher in thrombocytopenic children than in non-thrombocytopenic children. T-cell activation, TNF-alpha and IFN-gamma signaling markers were inversely correlated with thrombocyte levels, consistent with a more pronounced cytokine storm syndrome. Thrombocytopenia was not associated with severity of MIS-C and no pathogenic variants were identified in HLH-related genes. This suggests that thrombocytopenia in MIS-C is not a feature of a more severe disease phenotype, but the consequence of a distinct hyperinflammatory immunopathological process in a subset of children.

Original languageEnglish
Article number110252
JournalClinical Immunology
Volume264
Early online date17 May 2024
DOIs
Publication statusPublished - Jul 2024

Keywords

  • COVID-19
  • HLH
  • Immune dysregulation
  • MIS-C
  • SARS-CoV-2

Fingerprint

Dive into the research topics of 'Serum proteomics reveals hemophagocytic lymphohistiocytosis-like phenotype in a subset of patients with multisystem inflammatory syndrome in children'. Together they form a unique fingerprint.

Cite this