Serine aspartate repeat protein D increases Staphylococcus aureus virulence and survival in blood

Fatemeh Askarian*, Satoshi Uchiyama, J. Andrés Valderrama, Clement Ajayi, Johanna U E Sollid, Nina M. van Sorge, Victor Nizet, Jos A G van Strijp, Mona Johannessen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Staphylococcus aureus expresses a panel of cell wall-anchored adhesins, including proteins belonging to the microbial surface components recognizing adhesive matrix molecule (MSCRAMM) family, exemplified by the serine-aspartate repeat protein D (SdrD), which serve key roles in colonization and infection. Deletion of sdrD from S. aureus subsp. aureus strain NCTC8325-4 attenuated bacterial survival in human whole blood ex vivo, which was associated with increased killing by human neutrophils. Remarkably, SdrD was able to inhibit innate immune-mediated bacterial killing independently of other S. aureus proteins, since addition of recombinant SdrD protein and heterologous expression of SdrD in Lactococcus lactis promoted bacterial survival in human blood. SdrD contributes to bacterial virulence in vivo, since fewer S. aureus subsp. aureus NCTC8325-4 ΔsdrD bacteria than bacteria of the parent strain were recovered from blood and several organs using a murine intravenous infection model. Collectively, our findings reveal a new property of SdrD as an important key contributor to S. aureus survival and the ability to escape the innate immune system in blood.

Original languageEnglish
Article numbere00559-16
JournalInfection and Immunity
Volume85
Issue number1
DOIs
Publication statusPublished - 2017

Keywords

  • Neutrophils
  • SdrD
  • Systemic infection
  • Virulence
  • Whole blood

Fingerprint

Dive into the research topics of 'Serine aspartate repeat protein D increases Staphylococcus aureus virulence and survival in blood'. Together they form a unique fingerprint.

Cite this