TY - JOUR
T1 - Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
AU - Lleras Forero, Laura
AU - Narayanan, Rachna
AU - Huitema, Leonie F.A.
AU - Vanbergen, Maaike
AU - Apschner, Alexander
AU - Peterson-Maduro, Josi
AU - Logister, Ive
AU - Valentin, Guillaume
AU - Morelli, Luis G.
AU - Oates, Andrew C.
AU - Schulte-Merker, Stefan
N1 - Funding Information:
We thank the Schulte-Merker and Oates groups for constructive discussions. The Institute of Biostatistics and Clinical Research University of Münster provided helpful insights, the IT-Zentrum For-schung und Lehre (WWU Münster) rendered IT support. Numerous technicians and part time students helped in genotyping and maintaining zebrafish lines. Prof. J den Hertog provided the her1, her7, tbx6, aei and bea mutant lines to SSM, Prof. K Kawakami the Sagff214:galFF strain. This study was also funded by The Francis Crick Institute, receiving its core funding from Cancer Research UK, the Medical Research Council, and Wellcome to RN and ACO.
Funding Information:
We thank the Schulte-Merker and Oates groups for constructive discussions. The Institute of Biosta-tistics and Clinical Research University of Münster provided helpful insights, the IT-Zentrum For-schung und Lehre (WWU Münster) rendered IT support. Numerous technicians and part time students helped in genotyping and maintaining zebrafish lines. Prof. J den Hertog provided the her1, her7, tbx6, aei and bea mutant lines to SSM, Prof. K Kawakami the Sagff214:galFF strain. This study was also funded by The Francis Crick Institute, receiving its core funding from Cancer Research UK, the Medical Research Council, and Wellcome to RN and ACO. Deutsche Forschungsgemeinschaft CIM1003 Laura Lleras Forero Stefan Schulte-Merker Medical Research Council MC_UP_1202/3 Rachna Narayanan Andrew C Oates Francis Crick Institute Rachna Narayanan Andrew C Oates Wellcome WT098025MA uillaume Valentin Andrew C Oates Fondo Para la Convergencia Estructural del MERCOSUR COF 3/11 Luis G Morelli Agencia Nacional de Promoción Científica y Tecnológica PICT 2012 1954 Luis G Morelli Agencia Nacional de Promoción Científica y Tecnológica PICT 2013 1301 Luis G Morelli.
Publisher Copyright:
© Lleras Forero et al.
PY - 2018/4/6
Y1 - 2018/4/6
N2 - Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
AB - Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
UR - http://www.scopus.com/inward/record.url?scp=85046860010&partnerID=8YFLogxK
U2 - 10.7554/eLife.33843
DO - 10.7554/eLife.33843
M3 - Article
C2 - 29624170
AN - SCOPUS:85046860010
SN - 2050-084X
VL - 7
JO - eLife
JF - eLife
M1 - e33843
ER -