Safety Of Electro-Oxidation For Urea Removal In A Wearable Artificial Kidney Is Compromised By Formation Of Glucose Degradation Products

Maaike K van Gelder, Jeroen C Vollenbroek, Babette H Lentferink, Diënty H M Hazenbrink, Paul J Besseling, Frank Simonis, Silvia Giovanella, Giulia Ligabue, Maria A Bajo Rubio, Gianni Cappelli, Jaap A Joles, Marianne C Verhaar, Karin G F Gerritsen

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Abstract

A major challenge for the development of a wearable artificial kidney (WAK) is the removal of urea from the spent dialysate, as urea is the waste solute with the highest daily molar production and is difficult to adsorb. Here we present results on glucose degradation products (GDPs) formed during electrooxidation (EO), a technique that applies a current to the dialysate to convert urea into nitrogen, carbon dioxide, and hydrogen gas. Uremic plasma and peritoneal effluent were dialyzed for 8 hours with a WAK with and without EO-based dialysate regeneration. Samples were taken regularly during treatment. GDPs (glyoxal, methylglyoxal, and 3-deoxyglucosone) were measured in EO- and non-EO-treated fluids. Glyoxal and methylglyoxal concentrations increased 26- and 11-fold, respectively, in uremic plasma (at [glucose] 7 mmol/L) and 209- and 353-fold, respectively, in peritoneal effluent (at [glucose] 100 mmol/L) during treatment with EO, whereas no change was observed in GDP concentrations during dialysate regeneration without EO. EO for dialysate regeneration in a WAK is currently not safe due to the generation of GDPs which are not biocompatible.

Original languageEnglish
Pages (from-to)1422-1428
Number of pages7
JournalArtificial Organs
Volume45
Issue number11
Early online date23 Jul 2021
DOIs
Publication statusPublished - Nov 2021

Keywords

  • 3-Deoxyglucosone
  • artificial kidney
  • biocompatibility
  • electrooxidation
  • glucose degradation products
  • glyoxal
  • hemodialysis
  • methylglyoxal
  • peritoneal dialysis
  • urea

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