Routine identification of Nocardia species by MALDI-TOF mass spectrometry

Victoria Girard; Sandrine Mailler; Sophie Polsinelli; Delphine Jacob; Marie Christine Saccomani; Beatrice Celliere; Valerie Monnin; Alex van Belkum; Ferry Hagen; Jacques F Meis; Géraldine Durand

doi:10.1016/j.diagmicrobio.2016.09.024

Routine identification of Nocardia species by MALDI-TOF mass spectrometry

Victoria Girard^*
, Sandrine Mailler
, Sophie Polsinelli
, Delphine Jacob
, Marie Christine Saccomani
, Beatrice Celliere
, Valerie Monnin
, Alex van Belkum
, Ferry Hagen
, Jacques F Meis
, Géraldine Durand

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).

Original language	English
Pages (from-to)	7-10
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	87
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2016.09.024
Publication status	Published - Jan 2017
Externally published	Yes

Keywords

Bacteriological Techniques/methods
Humans
Nocardia Infections/diagnosis
Nocardia/chemistry
Specimen Handling/methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods

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10.1016/j.diagmicrobio.2016.09.024

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title = "Routine identification of Nocardia species by MALDI-TOF mass spectrometry",

abstract = "We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94\% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).",

keywords = "Bacteriological Techniques/methods, Humans, Nocardia Infections/diagnosis, Nocardia/chemistry, Specimen Handling/methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods",

author = "Victoria Girard and Sandrine Mailler and Sophie Polsinelli and Delphine Jacob and Saccomani, \{Marie Christine\} and Beatrice Celliere and Valerie Monnin and \{van Belkum\}, Alex and Ferry Hagen and Meis, \{Jacques F\} and G{\'e}raldine Durand",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

year = "2017",

month = jan,

doi = "10.1016/j.diagmicrobio.2016.09.024",

language = "English",

volume = "87",

pages = "7--10",

journal = "Diagnostic Microbiology and Infectious Disease",

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publisher = "Elsevier",

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TY - JOUR

T1 - Routine identification of Nocardia species by MALDI-TOF mass spectrometry

AU - Girard, Victoria

AU - Mailler, Sandrine

AU - Polsinelli, Sophie

AU - Jacob, Delphine

AU - Saccomani, Marie Christine

AU - Celliere, Beatrice

AU - Monnin, Valerie

AU - van Belkum, Alex

AU - Hagen, Ferry

AU - Meis, Jacques F

AU - Durand, Géraldine

PY - 2017/1

Y1 - 2017/1

N2 - We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).

AB - We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).

KW - Bacteriological Techniques/methods

KW - Humans

KW - Nocardia Infections/diagnosis

KW - Nocardia/chemistry

KW - Specimen Handling/methods

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods

U2 - 10.1016/j.diagmicrobio.2016.09.024

DO - 10.1016/j.diagmicrobio.2016.09.024

M3 - Article

C2 - 27802877

SN - 0732-8893

VL - 87

SP - 7

EP - 10

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Routine identification of Nocardia species by MALDI-TOF mass spectrometry

Abstract

Keywords

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