Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells

K.A. Smith; S. Chocron; S. von der Hardt; E.M. de Pater; A. Soufan; J. Bussmann; S. Schulte-Merker; M. Hammerschmidt; J. Bakkers

Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells

Translated title of the contribution: Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells

K.A. Smith, S. Chocron, S. von der Hardt, E.M. de Pater, A. Soufan, J. Bussmann, S. Schulte-Merker, M. Hammerschmidt, J. Bakkers

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We have used high-resolution 4D imaging of cardiac progenitor cells (CPCs) in zebrafish to investigate the earliest left-right asymmetric movements during cardiac morphogenesis. Differential migratory behavior within the heart field was observed, resulting in a rotation of the heart tube. The leftward displacement and rotation of the tube requires hyaluronan synthase 2 expression within the CPCs. Furthermore, by reducing or ectopically activating BMP signaling or by implantation of BMP beads we could demonstrate that BMP signaling, which is asymmetrically activated in the lateral plate mesoderm and regulated by early left-right signals, is required to direct CPC migration and cardiac rotation. Together, these results support a model in which CPCs migrate toward a BMP source during development of the linear heart tube, providing a mechanism by which the left-right axis drives asymmetric development of the vertebrate heart

Translated title of the contribution	Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells
Original language	Undefined/Unknown
Pages (from-to)	287-297
Number of pages	11
Journal	Developmental Cell
Volume	14
Issue number	2
Publication status	Published - 2008

Cite this

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title = "Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells",

abstract = "We have used high-resolution 4D imaging of cardiac progenitor cells (CPCs) in zebrafish to investigate the earliest left-right asymmetric movements during cardiac morphogenesis. Differential migratory behavior within the heart field was observed, resulting in a rotation of the heart tube. The leftward displacement and rotation of the tube requires hyaluronan synthase 2 expression within the CPCs. Furthermore, by reducing or ectopically activating BMP signaling or by implantation of BMP beads we could demonstrate that BMP signaling, which is asymmetrically activated in the lateral plate mesoderm and regulated by early left-right signals, is required to direct CPC migration and cardiac rotation. Together, these results support a model in which CPCs migrate toward a BMP source during development of the linear heart tube, providing a mechanism by which the left-right axis drives asymmetric development of the vertebrate heart",

author = "K.A. Smith and S. Chocron and {von der Hardt}, S. and {de Pater}, E.M. and A. Soufan and J. Bussmann and S. Schulte-Merker and M. Hammerschmidt and J. Bakkers",

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T1 - Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells

AU - Smith, K.A.

AU - Chocron, S.

AU - von der Hardt, S.

AU - de Pater, E.M.

AU - Soufan, A.

AU - Bussmann, J.

AU - Schulte-Merker, S.

AU - Hammerschmidt, M.

AU - Bakkers, J.

N1 - J EB

PY - 2008

Y1 - 2008

N2 - We have used high-resolution 4D imaging of cardiac progenitor cells (CPCs) in zebrafish to investigate the earliest left-right asymmetric movements during cardiac morphogenesis. Differential migratory behavior within the heart field was observed, resulting in a rotation of the heart tube. The leftward displacement and rotation of the tube requires hyaluronan synthase 2 expression within the CPCs. Furthermore, by reducing or ectopically activating BMP signaling or by implantation of BMP beads we could demonstrate that BMP signaling, which is asymmetrically activated in the lateral plate mesoderm and regulated by early left-right signals, is required to direct CPC migration and cardiac rotation. Together, these results support a model in which CPCs migrate toward a BMP source during development of the linear heart tube, providing a mechanism by which the left-right axis drives asymmetric development of the vertebrate heart

AB - We have used high-resolution 4D imaging of cardiac progenitor cells (CPCs) in zebrafish to investigate the earliest left-right asymmetric movements during cardiac morphogenesis. Differential migratory behavior within the heart field was observed, resulting in a rotation of the heart tube. The leftward displacement and rotation of the tube requires hyaluronan synthase 2 expression within the CPCs. Furthermore, by reducing or ectopically activating BMP signaling or by implantation of BMP beads we could demonstrate that BMP signaling, which is asymmetrically activated in the lateral plate mesoderm and regulated by early left-right signals, is required to direct CPC migration and cardiac rotation. Together, these results support a model in which CPCs migrate toward a BMP source during development of the linear heart tube, providing a mechanism by which the left-right axis drives asymmetric development of the vertebrate heart

M3 - Article

SN - 1534-5807

VL - 14

SP - 287

EP - 297

JO - Developmental Cell

JF - Developmental Cell

IS - 2

ER -

Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells

Abstract

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