@article{7f22c1e72fa74f7494bf52f4f159199d,
title = "Role of the cellular factor CTCF in the regulation of bovine leukemia virus latency and three-dimensional chromatin organization",
abstract = "Bovine leukemia virus (BLV)-induced tumoral development is a multifactorial phenomenon that remains incompletely understood. Here, we highlight the critical role of the cellular CCCTC-binding factor (CTCF) both in the regulation of BLV transcriptional activities and in the deregulation of the three-dimensional (3D) chromatin architecture surrounding the BLV integration site. We demonstrated the in vivo recruitment of CTCF to three conserved CTCF binding motifs along the provirus. Next, we showed that CTCF localized to regions of transitions in the histone modifications profile along the BLV genome and that it is implicated in the repression of the 5′Long Terminal Repeat (LTR) promoter activity, thereby contributing to viral latency, while favoring the 3′LTR promoter activity. Finally, we demonstrated that BLV integration deregulated the host cellular 3D chromatin organization through the formation of viral/host chromatin loops. Altogether, our results highlight CTCF as a new critical effector of BLV transcriptional regulation and BLV-induced physiopathology. ",
keywords = "CCCTC-Binding Factor/metabolism, Chromatin, Leukemia Virus, Bovine/genetics, Promoter Regions, Genetic, Terminal Repeat Sequences/genetics, Virus Latency",
author = "Maxime Bellefroid and Anthony Rodari and Mathilde Galais and Krijger, {Peter H.L.} and Tjalsma, {Sjoerd J.D.} and Lorena Nestola and Estelle Plant and Vos, {Erica S.M.} and Sara Cristinelli and {Van Driessche}, Benoit and Caroline Vanhulle and Amina Ait-Ammar and Ars{\`e}ne Burny and Angela Ciuffi and {De Laat}, Wouter and {Van Lint}, Carine",
note = "Funding Information: C.V.L. acknowledges funding from the Belgian National Fund for Scientific Research (F.R.S-FNRS, Belgium); Fondation Roi Baudouin; Internationale Brachet Stiftung (IBS);WalloonRegion (Fonds deMaturation); Les Amis des Instituts Pasteur `a Bruxelles, asbl.; University of Brussels (ULB - Action de Recherche Concert{\'e}e (ARC) grant); 'cr{\'e}dit de recherche' grant of the F.R.S.- FNRS [J.0021.17]; Swiss National Science Foundation [314730 188877]; the laboratory of C.V.L. is part of the ULB-Cancer Research Centre (U-CRC); A.R. is a postdoctoral fellow (ULB ARC program); M.B. is a doctoral fellow from the Belgian 'Fonds pour la formation `a la Recherche dans l'Industrie et dans l'Agriculture (FRIA)'; M.G. is a postdoctoral fellow supported by 'Les Amis des Instituts Pasteur `a Bruxelles, asbl'; L.N. is supported by a 'projet de recherche' grant from the F.R.S.-FNRS; E.P. is a doctoral fellow from the Tel{\'e}vie program of the F.R.S.-FNRS; W.D.L. is founder and shareholder of Cergentis; C.V.L. is 'Directeur de Recherches' of the F.R.S.- FNRS. Published with the support of the {"}Fondation Universitaire de Belgique{"} Publisher Copyright: {\textcopyright} 2022 The Author(s).",
year = "2022",
month = apr,
day = "8",
doi = "10.1093/nar/gkac107",
language = "English",
volume = "50",
pages = "3190--3202",
journal = "Nucleic acids research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "6",
}