Abstract
Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation.
Translated title of the contribution | Robust 4C-seq data analysis to screen for regulatory DNA interactions. |
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Original language | Undefined/Unknown |
Pages (from-to) | 969-72 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 9 |
Issue number | 10 |
Publication status | Published - 2012 |