Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution"

Amanda R Dos Santos; Alexandro Bonifaz; Amanda Bombassaro; Ana Caroline de Sá Machado; Anderson M Rodrigues; Andrew M Borman; Arunaloke Chakrabarti; Anuradha Chowdary; Bruno Rediguieri; Elizabeth M Johnson; Ferry Hagen; Flavio Queiroz-Telles; Gloria M Gonzalez; Guillermo Garcia-Effron; Isabella Dib Ferreira Gremião; Jacques F Meis; Juliana Possato Fernandes Takahashi; Luana Pereira Borba Dos Santos; Marcia S C Melhem; Paola Cappellano; Raimunda Sâmia Nogueira Brilhante; Sandro Antonio Pereira; Sarah Santos Gonçalvez; Sarah Kidd; Sean X Zhang; Shivaprakash Rudramurthy; Sonia Rozental; Susana Cordoba; Theun de Groot; Wei Liu; Nathan P Wiederhold; Eelco F J Meijer; Shawn R Lockhart; Philippe J Dufresne

doi:10.1128/aac.00566-26

Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution"

Amanda R Dos Santos^*
, Alexandro Bonifaz
, Amanda Bombassaro
, Ana Caroline de Sá Machado
, Anderson M Rodrigues
, Andrew M Borman
, Arunaloke Chakrabarti
, Anuradha Chowdary
, Bruno Rediguieri
, Elizabeth M Johnson
, Ferry Hagen
, Flavio Queiroz-Telles
, Gloria M Gonzalez
, Guillermo Garcia-Effron
, Isabella Dib Ferreira Gremião
, Jacques F Meis
, Juliana Possato Fernandes Takahashi
, Luana Pereira Borba Dos Santos
, Marcia S C Melhem
, Paola Cappellano

Raimunda Sâmia Nogueira Brilhante, Sandro Antonio Pereira, Sarah Santos Gonçalvez, Sarah Kidd, Sean X Zhang, Shivaprakash Rudramurthy, Sonia Rozental, Susana Cordoba, Theun de Groot, Wei Liu, Nathan P Wiederhold, Eelco F J Meijer, Shawn R Lockhart, Philippe J Dufresne

^*Corresponding author for this work

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

Original language	English
Article number	e0056626
Journal	Antimicrobial Agents and Chemotherapy
Volume	70
Issue number	8
Early online date	24 Jun 2026
DOIs	https://doi.org/10.1128/aac.00566-26
Publication status	Published - 5 Aug 2026

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dos-santos-et-al-2026-reply-to-chen-et-al-methodological-considerations-for-the-establishment-of-epidemiological-cutoffFinal published version, 343 KBLicence: CC BY

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Dos Santos, A. R., Bonifaz, A., Bombassaro, A., de Sá Machado, A. C., Rodrigues, A. M., Borman, A. M., Chakrabarti, A., Chowdary, A., Rediguieri, B., Johnson, E. M., Hagen, F., Queiroz-Telles, F., Gonzalez, G. M., Garcia-Effron, G., Dib Ferreira Gremião, I., Meis, J. F., Possato Fernandes Takahashi, J., Pereira Borba Dos Santos, L., Melhem, M. S. C., ... Dufresne, P. J. (2026). Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution". Antimicrobial Agents and Chemotherapy, 70(8), Article e0056626. https://doi.org/10.1128/aac.00566-26

@article{c9b4105b2a3541a0bacd7f05789698e3,

title = "Reply to Chen et al., {"}Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution{"}",

author = "\{Dos Santos\}, \{Amanda R\} and Alexandro Bonifaz and Amanda Bombassaro and \{de S{\'a} Machado\}, \{Ana Caroline\} and Rodrigues, \{Anderson M\} and Borman, \{Andrew M\} and Arunaloke Chakrabarti and Anuradha Chowdary and Bruno Rediguieri and Johnson, \{Elizabeth M\} and Ferry Hagen and Flavio Queiroz-Telles and Gonzalez, \{Gloria M\} and Guillermo Garcia-Effron and \{Dib Ferreira Gremi{\~a}o\}, Isabella and Meis, \{Jacques F\} and \{Possato Fernandes Takahashi\}, Juliana and \{Pereira Borba Dos Santos\}, Luana and Melhem, \{Marcia S C\} and Paola Cappellano and \{Nogueira Brilhante\}, \{Raimunda S{\^a}mia\} and Pereira, \{Sandro Antonio\} and \{Santos Gon{\c c}alvez\}, Sarah and Sarah Kidd and Zhang, \{Sean X\} and Shivaprakash Rudramurthy and Sonia Rozental and Susana Cordoba and \{de Groot\}, Theun and Wei Liu and Wiederhold, \{Nathan P\} and Meijer, \{Eelco F J\} and Lockhart, \{Shawn R\} and Dufresne, \{Philippe J\}",

year = "2026",

month = aug,

day = "5",

doi = "10.1128/aac.00566-26",

language = "English",

volume = "70",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "8",

}

Dos Santos, AR, Bonifaz, A, Bombassaro, A, de Sá Machado, AC, Rodrigues, AM, Borman, AM, Chakrabarti, A, Chowdary, A, Rediguieri, B, Johnson, EM, Hagen, F, Queiroz-Telles, F, Gonzalez, GM, Garcia-Effron, G, Dib Ferreira Gremião, I, Meis, JF, Possato Fernandes Takahashi, J, Pereira Borba Dos Santos, L, Melhem, MSC, Cappellano, P, Nogueira Brilhante, RS, Pereira, SA, Santos Gonçalvez, S, Kidd, S, Zhang, SX, Rudramurthy, S, Rozental, S, Cordoba, S, de Groot, T, Liu, W, Wiederhold, NP, Meijer, EFJ, Lockhart, SR & Dufresne, PJ 2026, 'Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution"', Antimicrobial Agents and Chemotherapy, vol. 70, no. 8, e0056626. https://doi.org/10.1128/aac.00566-26

Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution". / Dos Santos, Amanda R; Bonifaz, Alexandro; Bombassaro, Amanda et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 70, No. 8, e0056626, 05.08.2026.

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

TY - JOUR

T1 - Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution"

AU - Dos Santos, Amanda R

AU - Bonifaz, Alexandro

AU - Bombassaro, Amanda

AU - de Sá Machado, Ana Caroline

AU - Rodrigues, Anderson M

AU - Borman, Andrew M

AU - Chakrabarti, Arunaloke

AU - Chowdary, Anuradha

AU - Rediguieri, Bruno

AU - Johnson, Elizabeth M

AU - Hagen, Ferry

AU - Queiroz-Telles, Flavio

AU - Gonzalez, Gloria M

AU - Garcia-Effron, Guillermo

AU - Dib Ferreira Gremião, Isabella

AU - Meis, Jacques F

AU - Possato Fernandes Takahashi, Juliana

AU - Pereira Borba Dos Santos, Luana

AU - Melhem, Marcia S C

AU - Cappellano, Paola

AU - Nogueira Brilhante, Raimunda Sâmia

AU - Pereira, Sandro Antonio

AU - Santos Gonçalvez, Sarah

AU - Kidd, Sarah

AU - Zhang, Sean X

AU - Rudramurthy, Shivaprakash

AU - Rozental, Sonia

AU - Cordoba, Susana

AU - de Groot, Theun

AU - Liu, Wei

AU - Wiederhold, Nathan P

AU - Meijer, Eelco F J

AU - Lockhart, Shawn R

AU - Dufresne, Philippe J

PY - 2026/8/5

Y1 - 2026/8/5

UR - https://www.scopus.com/pages/publications/105046475548

U2 - 10.1128/aac.00566-26

DO - 10.1128/aac.00566-26

M3 - Comment/Letter to the editor

C2 - 42340338

SN - 0066-4804

VL - 70

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 8

M1 - e0056626

ER -

Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution"

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