Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.

P. Knipscheer; M. Raschle; O.D. Scharer; J.C. Walter

Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.

Translated title of the contribution: Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.

P. Knipscheer
, M. Raschle
, O.D. Scharer
, J.C. Walter

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

Translated title of the contribution	Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.
Original language	Undefined/Unknown
Pages (from-to)	221-43
Number of pages	23
Journal	Methods in molecular biology
Volume	920
Publication status	Published - 2012

Cite this

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title = "Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.",

abstract = "Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.",

author = "P. Knipscheer and M. Raschle and O.D. Scharer and J.C. Walter",

year = "2012",

language = "Undefined/Unknown",

volume = "920",

pages = "221--43",

journal = "Methods in molecular biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

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TY - JOUR

T1 - Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.

AU - Knipscheer, P.

AU - Raschle, M.

AU - Scharer, O.D.

AU - Walter, J.C.

PY - 2012

Y1 - 2012

N2 - Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

AB - Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

M3 - Article

SN - 1064-3745

VL - 920

SP - 221

EP - 243

JO - Methods in molecular biology

JF - Methods in molecular biology

ER -

Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.

Abstract

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