Regulated expression of the X. tropicalis connexin43 promoter

Marcel A.G. Van Der Heyden; Liesbeth Roeleveld; Stefan Reneman; Josi Peterson; Olivier H.J. Destrée

Regulated expression of the X. tropicalis connexin43 promoter

Marcel A.G. Van Der Heyden, Liesbeth Roeleveld, Stefan Reneman, Josi Peterson, Olivier H.J. Destrée^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

The spatio-temporal expression pattern of the connexin43 gene during Xenopus development has been described (Van der Heyden et al. 2001). To further investigate the regulation and function of connexin43 (Cx43) in amphibians, we have isolated the gene from Xenopus tropicalis (Xt) and determined its structure. The X. tropicalis Cx43 gene displays the typical two exon-one intron connexin configuration, where the first exon is non-coding. The predicted amino acid sequence of the XtCx43 protein is highly homologous to that of X. laevis, chicken and mammals. Expression of XtCx43 cDNA in N2A cells results in gap-junction plaque formation. Promoter activity of a 3.5 kb upstream region of the X. tropicalis Cx43 gene, including exon 1, mimics endogenous timing of expression after injection of reporter constructs in X. laevis embryos.

Original language	English
Pages (from-to)	293-298
Number of pages	6
Journal	Cell Communication and Adhesion
Volume	8
Issue number	4-6
Publication status	Published - 1 Dec 2001

Keywords

Connexin
Gap junction
N2A
Promoter
Xenopus (silurana) tropicalis
Xenopus laevis

Cite this

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title = "Regulated expression of the X. tropicalis connexin43 promoter",

abstract = "The spatio-temporal expression pattern of the connexin43 gene during Xenopus development has been described (Van der Heyden et al. 2001). To further investigate the regulation and function of connexin43 (Cx43) in amphibians, we have isolated the gene from Xenopus tropicalis (Xt) and determined its structure. The X. tropicalis Cx43 gene displays the typical two exon-one intron connexin configuration, where the first exon is non-coding. The predicted amino acid sequence of the XtCx43 protein is highly homologous to that of X. laevis, chicken and mammals. Expression of XtCx43 cDNA in N2A cells results in gap-junction plaque formation. Promoter activity of a 3.5 kb upstream region of the X. tropicalis Cx43 gene, including exon 1, mimics endogenous timing of expression after injection of reporter constructs in X. laevis embryos.",

keywords = "Connexin, Gap junction, N2A, Promoter, Xenopus (silurana) tropicalis, Xenopus laevis",

author = "{Van Der Heyden}, {Marcel A.G.} and Liesbeth Roeleveld and Stefan Reneman and Josi Peterson and Destr{\'e}e, {Olivier H.J.}",

year = "2001",

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language = "English",

volume = "8",

pages = "293--298",

number = "4-6",

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TY - JOUR

T1 - Regulated expression of the X. tropicalis connexin43 promoter

AU - Van Der Heyden, Marcel A.G.

AU - Roeleveld, Liesbeth

AU - Reneman, Stefan

AU - Peterson, Josi

AU - Destrée, Olivier H.J.

PY - 2001/12/1

Y1 - 2001/12/1

N2 - The spatio-temporal expression pattern of the connexin43 gene during Xenopus development has been described (Van der Heyden et al. 2001). To further investigate the regulation and function of connexin43 (Cx43) in amphibians, we have isolated the gene from Xenopus tropicalis (Xt) and determined its structure. The X. tropicalis Cx43 gene displays the typical two exon-one intron connexin configuration, where the first exon is non-coding. The predicted amino acid sequence of the XtCx43 protein is highly homologous to that of X. laevis, chicken and mammals. Expression of XtCx43 cDNA in N2A cells results in gap-junction plaque formation. Promoter activity of a 3.5 kb upstream region of the X. tropicalis Cx43 gene, including exon 1, mimics endogenous timing of expression after injection of reporter constructs in X. laevis embryos.

AB - The spatio-temporal expression pattern of the connexin43 gene during Xenopus development has been described (Van der Heyden et al. 2001). To further investigate the regulation and function of connexin43 (Cx43) in amphibians, we have isolated the gene from Xenopus tropicalis (Xt) and determined its structure. The X. tropicalis Cx43 gene displays the typical two exon-one intron connexin configuration, where the first exon is non-coding. The predicted amino acid sequence of the XtCx43 protein is highly homologous to that of X. laevis, chicken and mammals. Expression of XtCx43 cDNA in N2A cells results in gap-junction plaque formation. Promoter activity of a 3.5 kb upstream region of the X. tropicalis Cx43 gene, including exon 1, mimics endogenous timing of expression after injection of reporter constructs in X. laevis embryos.

KW - Connexin

KW - Gap junction

KW - N2A

KW - Promoter

KW - Xenopus (silurana) tropicalis

KW - Xenopus laevis

UR - http://www.scopus.com/inward/record.url?scp=0035754422&partnerID=8YFLogxK

M3 - Article

C2 - 12064605

AN - SCOPUS:0035754422

SN - 1061-5385

VL - 8

SP - 293

EP - 298

JO - Cell Communication and Adhesion

JF - Cell Communication and Adhesion

IS - 4-6

ER -

Regulated expression of the X. tropicalis connexin43 promoter

Abstract

Keywords

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