Reduced Expression of HLA-DR on Monocytes During Severe Respiratory Syncytial Virus Infections

Inge M L Ahout; Jop Jans; Lilid Haroutiounian; Elles R Simonetti; Christa van der Gaast-de Jongh; Dimitri A Diavatopoulos; Marien I de Jonge; Ronald de Groot; Gerben Ferwerda

doi:10.1097/INF.0000000000001007

Reduced Expression of HLA-DR on Monocytes During Severe Respiratory Syncytial Virus Infections

Inge M L Ahout, Jop Jans, Lilid Haroutiounian, Elles R Simonetti, Christa van der Gaast-de Jongh, Dimitri A Diavatopoulos, Marien I de Jonge, Ronald de Groot, Gerben Ferwerda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Respiratory syncytial virus (RSV) is a common cause of bronchiolitis in infants with a wide spectrum of disease severity. Besides environmental and genetic factors, it is thought that the innate immune system plays a pivotal role. The aim of this study was to investigate the expression of immune receptors on monocytes and the in vitro responsiveness from infants with severe RSV infections.

METHODS: Peripheral blood mononuclear cells (PBMCs) from infants with RSV infections were isolated. Classical, intermediate and nonclassical monocytes were immunophenotyped for the expression of CD14, CD16, human leukocyte antigen (HLA)-ABC and HLA-DR. PBMCs were stimulated with lipopolysaccharide to determine the secretion of tumor necrosis factor and interleukin (IL)-10 with enzyme-linked immunosorbent assay.

RESULTS: During RSV infection, intermediate monocytes are increased in the peripheral blood, whereas classical and nonclassical monocytes are reduced. The expression of CD14 and HLA-ABC is increased on monocytes, whereas the expression of HLA-DR is suppressed. Low HLA-DR expression is correlated with increased disease severity. PBMCs from infants with severe RSV infections show an impaired IL-10 response in vitro.

CONCLUSIONS: Phenotyping subpopulations of monocytes combined with in vitro responsiveness reveals significant differences between nonsevere and severe RSV infections. Reduced HLA-DR expression and impaired IL-10 production in vitro during severe RSV infections indicate that an imbalanced innate immune response may play an important role in disease severity.

Original language	English
Pages (from-to)	e89-96
Journal	The Pediatric infectious disease journal
Volume	35
Issue number	3
DOIs	https://doi.org/10.1097/INF.0000000000001007
Publication status	Published - Mar 2016
Externally published	Yes

Keywords

Biomarkers
Case-Control Studies
Cohort Studies
Female
Gene Expression
HLA-DR Antigens/genetics
Humans
Immunophenotyping
Infant
Interleukin-10/biosynthesis
Leukocyte Count
Leukocytes, Mononuclear/immunology
Male
Monocytes/immunology
Phenotype
Respiratory Syncytial Virus Infections/diagnosis
Respiratory Syncytial Virus, Human/immunology
Risk Factors
Severity of Illness Index

Access to Document

10.1097/INF.0000000000001007

Cite this

@article{1a44c526c1c247359fdf1aec54abd4a8,

title = "Reduced Expression of HLA-DR on Monocytes During Severe Respiratory Syncytial Virus Infections",

abstract = "BACKGROUND: Respiratory syncytial virus (RSV) is a common cause of bronchiolitis in infants with a wide spectrum of disease severity. Besides environmental and genetic factors, it is thought that the innate immune system plays a pivotal role. The aim of this study was to investigate the expression of immune receptors on monocytes and the in vitro responsiveness from infants with severe RSV infections.METHODS: Peripheral blood mononuclear cells (PBMCs) from infants with RSV infections were isolated. Classical, intermediate and nonclassical monocytes were immunophenotyped for the expression of CD14, CD16, human leukocyte antigen (HLA)-ABC and HLA-DR. PBMCs were stimulated with lipopolysaccharide to determine the secretion of tumor necrosis factor and interleukin (IL)-10 with enzyme-linked immunosorbent assay.RESULTS: During RSV infection, intermediate monocytes are increased in the peripheral blood, whereas classical and nonclassical monocytes are reduced. The expression of CD14 and HLA-ABC is increased on monocytes, whereas the expression of HLA-DR is suppressed. Low HLA-DR expression is correlated with increased disease severity. PBMCs from infants with severe RSV infections show an impaired IL-10 response in vitro.CONCLUSIONS: Phenotyping subpopulations of monocytes combined with in vitro responsiveness reveals significant differences between nonsevere and severe RSV infections. Reduced HLA-DR expression and impaired IL-10 production in vitro during severe RSV infections indicate that an imbalanced innate immune response may play an important role in disease severity.",

keywords = "Biomarkers, Case-Control Studies, Cohort Studies, Female, Gene Expression, HLA-DR Antigens/genetics, Humans, Immunophenotyping, Infant, Interleukin-10/biosynthesis, Leukocyte Count, Leukocytes, Mononuclear/immunology, Male, Monocytes/immunology, Phenotype, Respiratory Syncytial Virus Infections/diagnosis, Respiratory Syncytial Virus, Human/immunology, Risk Factors, Severity of Illness Index",

author = "Ahout, {Inge M L} and Jop Jans and Lilid Haroutiounian and Simonetti, {Elles R} and {van der Gaast-de Jongh}, Christa and Diavatopoulos, {Dimitri A} and {de Jonge}, {Marien I} and {de Groot}, Ronald and Gerben Ferwerda",

year = "2016",

month = mar,

doi = "10.1097/INF.0000000000001007",

language = "English",

volume = "35",

pages = "e89--96",

journal = "The Pediatric infectious disease journal",

issn = "0891-3668",

publisher = "Lippincott Williams & Wilkins",

number = "3",

}

TY - JOUR

T1 - Reduced Expression of HLA-DR on Monocytes During Severe Respiratory Syncytial Virus Infections

AU - Ahout, Inge M L

AU - Jans, Jop

AU - Haroutiounian, Lilid

AU - Simonetti, Elles R

AU - van der Gaast-de Jongh, Christa

AU - Diavatopoulos, Dimitri A

AU - de Jonge, Marien I

AU - de Groot, Ronald

AU - Ferwerda, Gerben

PY - 2016/3

Y1 - 2016/3

N2 - BACKGROUND: Respiratory syncytial virus (RSV) is a common cause of bronchiolitis in infants with a wide spectrum of disease severity. Besides environmental and genetic factors, it is thought that the innate immune system plays a pivotal role. The aim of this study was to investigate the expression of immune receptors on monocytes and the in vitro responsiveness from infants with severe RSV infections.METHODS: Peripheral blood mononuclear cells (PBMCs) from infants with RSV infections were isolated. Classical, intermediate and nonclassical monocytes were immunophenotyped for the expression of CD14, CD16, human leukocyte antigen (HLA)-ABC and HLA-DR. PBMCs were stimulated with lipopolysaccharide to determine the secretion of tumor necrosis factor and interleukin (IL)-10 with enzyme-linked immunosorbent assay.RESULTS: During RSV infection, intermediate monocytes are increased in the peripheral blood, whereas classical and nonclassical monocytes are reduced. The expression of CD14 and HLA-ABC is increased on monocytes, whereas the expression of HLA-DR is suppressed. Low HLA-DR expression is correlated with increased disease severity. PBMCs from infants with severe RSV infections show an impaired IL-10 response in vitro.CONCLUSIONS: Phenotyping subpopulations of monocytes combined with in vitro responsiveness reveals significant differences between nonsevere and severe RSV infections. Reduced HLA-DR expression and impaired IL-10 production in vitro during severe RSV infections indicate that an imbalanced innate immune response may play an important role in disease severity.

AB - BACKGROUND: Respiratory syncytial virus (RSV) is a common cause of bronchiolitis in infants with a wide spectrum of disease severity. Besides environmental and genetic factors, it is thought that the innate immune system plays a pivotal role. The aim of this study was to investigate the expression of immune receptors on monocytes and the in vitro responsiveness from infants with severe RSV infections.METHODS: Peripheral blood mononuclear cells (PBMCs) from infants with RSV infections were isolated. Classical, intermediate and nonclassical monocytes were immunophenotyped for the expression of CD14, CD16, human leukocyte antigen (HLA)-ABC and HLA-DR. PBMCs were stimulated with lipopolysaccharide to determine the secretion of tumor necrosis factor and interleukin (IL)-10 with enzyme-linked immunosorbent assay.RESULTS: During RSV infection, intermediate monocytes are increased in the peripheral blood, whereas classical and nonclassical monocytes are reduced. The expression of CD14 and HLA-ABC is increased on monocytes, whereas the expression of HLA-DR is suppressed. Low HLA-DR expression is correlated with increased disease severity. PBMCs from infants with severe RSV infections show an impaired IL-10 response in vitro.CONCLUSIONS: Phenotyping subpopulations of monocytes combined with in vitro responsiveness reveals significant differences between nonsevere and severe RSV infections. Reduced HLA-DR expression and impaired IL-10 production in vitro during severe RSV infections indicate that an imbalanced innate immune response may play an important role in disease severity.

KW - Biomarkers

KW - Case-Control Studies

KW - Cohort Studies

KW - Female

KW - Gene Expression

KW - HLA-DR Antigens/genetics

KW - Humans

KW - Immunophenotyping

KW - Infant

KW - Interleukin-10/biosynthesis

KW - Leukocyte Count

KW - Leukocytes, Mononuclear/immunology

KW - Male

KW - Monocytes/immunology

KW - Phenotype

KW - Respiratory Syncytial Virus Infections/diagnosis

KW - Respiratory Syncytial Virus, Human/immunology

KW - Risk Factors

KW - Severity of Illness Index

U2 - 10.1097/INF.0000000000001007

DO - 10.1097/INF.0000000000001007

M3 - Article

C2 - 26658377

SN - 0891-3668

VL - 35

SP - e89-96

JO - The Pediatric infectious disease journal

JF - The Pediatric infectious disease journal

IS - 3

ER -

Reduced Expression of HLA-DR on Monocytes During Severe Respiratory Syncytial Virus Infections

Abstract

Keywords

Access to Document

Fingerprint

Cite this