Rat gap junction connexin-30 inhibits proliferation of glioma cell lines

F Princen, P Robe, D Gros, T Jarry-Guichard, J Gielen, M P Merville, V Bours

Research output: Contribution to journalArticleAcademicpeer-review


Connexins, the structural components of gap junctions, control cell growth and differentiation and are believed to belong to a family of tumour suppressor genes. Studies on connexin localization in brain showed that several of these proteins were expressed in distinct compartments of the brain in a cell-type specific manner, indicating that different gap junctions play specific roles in the physiology of the mammalian brain. In this report, we first cloned rat connexin-30 cDNA from brain and showed that it was expressed in long-term primary culture of rat astrocytes. In order to examine the potential role of connexin-30 in tumour cell proliferation, we transfected the connexin-30 cDNA into two rat glioma cell lines (9L and C6) which have lost its expression. Transfected clones adequately expressed membrane-bound connexin-30 protein. Connexin-30-expressing clones showed slower growth, lower DNA synthesis and reduced proliferation in soft agar as compared with the parental and control cells. We concluded that connexin-30 may also probably be considered as a tumour suppressor in rat gliomas.

Original languageEnglish
Pages (from-to)507-13
Number of pages7
Issue number3
Publication statusPublished - Mar 2001


  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Connexin 30
  • Connexins/chemistry
  • DNA, Complementary
  • Gap Junctions/physiology
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rats
  • Sequence Homology, Amino Acid
  • Transfection
  • Tumor Cells, Cultured


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